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A Practical Guide to Frozen Section Technique

Editors: Peters, Stephen R.

DOI: 10.1007/978-1-4419-1234-3

ISBN: 978-1-4419-1233-6

Publisher: Springer New York - Springer Journals

Oct 23, 2009

1 - 9 of 9 Chapters

Available

Understanding and Maintaining the Cryostat

Hyam, Philip

Pages: 1 –11

[The chapter presents a user-friendly review of the main components of a routine cryostat and their function in the preparation of frozen sections of mammalian tissue. Topics covered include sectioning hints and tips and proper methods for disinfection and cryostat maintenance.]
Available

Gross Examination of Tissues in the Frozen Section Room

Peters, Stephen R.

Pages: 13 –35

[The chapter discusses gross examination of tissues in the setting of surgical pathology intraoperative consultation; the frozen section room. The process of gross examination is discussed in nine important steps from verifying of labeling and review of clinical information through gross...
Available

Embedding of Tissue for Frozen Section

Peters, Stephen R.

Pages: 37 –74

[Embedding of tissue for frozen sectioning is a vital step in the preparation of tissue for intra-operative consultation. In many cases, this task needs to be performed with great precision or risk suboptimal interpretation and loss of precious tissue. Traditional forms of face up and face down...
Available

Cutting the Frozen Section

Peters, Stephen R.

Pages: 75 –95

[The chapter covers a detailed method of cutting frozen sections on a cryostat using brush technique in continuous motion. The text covers all steps of the cutting process including trimming, cutting and retrieving. The goal is to develop great skill and sensitivity with the brush so that we can...
Available

Variables Affecting the Cutting Properties of Tissues and the Resulting Artifacts

Peters, Stephen R.

Pages: 97 –115

[This chapter elucidates the variables effecting the way tissues cut when preparing frozen sections. Our goal is to recognize artifacts on the sections as they are being cut and make corrections and adjustments to yield the best sections possible. The cutting properties of tissue with varying...
Available

Fixation, Staining and Coverslipping of Frozen Section Slides

Peters, Stephen R.; Delia, Catherine Susan

Pages: 117 –129

[This chapter covers fixation, staining and coverslipping of frozen section slides. A number of fixatives and fixative cocktails in use are described and illustrated as well as artifacts resulting from prolonged drying. Several commonly used staining procedures are described with suggestions on...
Available

Microscopic Interpretation in the Frozen Section Setting

Peters, Stephen R.

Pages: 131 –149

[There is no place in a surgical pathology practice that challenges our diagnostic skills more than the frozen section room. Given only a simple H&E section, under constraints of time and an anxious surgeon, we are faced with many diagnostic challenges. Our approach to read the frozen section...
Available

Frozen Section Techniques Used in Mohs Micrographic Surgery

Beck, Barbara; Peters, Stephen R.

Pages: 151 –169

[The chapter offers a comprehensive discussion of the technical process of preparing frozen sections in the setting of Mohs dermatographic surgery. In Mohs surgery procedure, the surgeon removes a skin cancer which is immediately examined by frozen section to access the resection margin. Most...
Available

Frozen Section Technique in the Animal Research Setting

Scouten, Charles W.

Pages: 171 –189

[There are significant differences between the skills required for research animal and for clinical histology. Researchers normally use sacrifice perfusion to harvest animal tissue, and need to freeze and section whole organs, sometimes in a specific orientation. Clinical histologists normally...

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