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Animal Cell Culture: Principles and Practice

Part of the Techniques in Life Science and Biomedicine for the Non-Expert Book Series

DOI: 10.1007/978-3-031-19485-6

ISBN: 978-3-031-19484-9

Publisher: Springer International Publishing - Springer Journals

Feb 1, 2023

1 - 10 of 21 Chapters

LitStream

Chapter 1Overview to Animal Cell Culture

Mani, Shalini

Pages: 1 –9

[The technique of taking cells from an animal or plant and growing them in an artificially controlled environment is known as cell culture. In many areas of the life sciences, cell culture has become a necessary tool. It lays the groundwork for researching cell proliferation, differentiation and...
LitStream

Chapter 2Cell Culture Laboratory

Jain, Anvi; Gulati, Aaru; Mittal, Khushi R.; Mani, Shalini

Pages: 11 –52

[The first objective of cell culture laboratory is to maintain hygiene and keep it contamination-free. The design of a cell culture laboratory majorly depends upon the type of activities performed. Therefore, it is preferred to have a designated work area to minimise the risk of contamination to...
LitStream

Chapter 3Good Laboratory Practices in Animal Cell Culture Laboratory and Biosafety Measures

Yukta, Kumari; Agarwal, Mansi; Pandey, Mekhla; Mittal, Khushi; Srivastava, Vidushi; Mani, Shalini

Pages: 53 –64

[Animal cell culture laboratory is considered to be one of the most sensitive labs, and it is important to keep these labs safe from any kind of contamination. Since animal cells are more prone to contaminations, it is advised to follow the proper procedures to attain desirable results. The goal...
LitStream

Chapter 4Managing Sterility in Animal Cell Culture Laboratory

Mani, Shalini

Pages: 65 –75

[Animal cell culture is the complex process by which cells are grown under controlled conditions. A successful cell culture profoundly depends on protecting the cells from exposure to all sources of biological contamination caused by various microorganisms such as bacteria, fungi and viruses;...
LitStream

Chapter 5Media and Buffer Preparation for Cell Culture

Tyagi, Sakshi; Mani, Shalini

Pages: 77 –88

[Cell culture is the most widely studied method in the field of life sciences. This term is generally used for the removal of cells, tissues or organs from a plant or an animal and their subsequent transfer into an artificial environment supporting their survival. The primary requirements for the...
LitStream

Chapter 6Properties of Cultured Cells and Selection of Culture Media

Mani, Shalini

Pages: 89 –97

[In vitro cultured cells possess discrete properties in comparison to the normal cells, and therefore, they require a suitable environmental system for their growth. The nature of different environmental factors significantly affects the cells in culture conditions, most commonly the kind of...
LitStream

Chapter 7Selection and Maintenance of Cultured Cells

Jindal, Divya; Singh, Manisha

Pages: 99 –114

[In the exploration of basic scientific and translational research concerns, cell culture is a very versatile technique. The uniformity of cell lines and the related reproducibility of information obtained are benefits of using them in scientific study. Because the readout of the desired assay...
LitStream

Chapter 8Inoculation and Passaging of Adherent and Suspension Cells

Pancham, Pranav; Jindal, Divya; Singh, Manisha

Pages: 115 –130

[Cell culture is an essential and necessary technique used in cell culture, unbiased with the growth of various organisms by introducing of various upcoming advancements in the field. The chapter describes the various possibilities and methods to inoculate adherent and suspension cell cultures to...
LitStream

Chapter 9Counting of Cells

Jindal, Divya; Singh, Manisha

Pages: 131 –145

[Counting cells is a common yet time-consuming process in in vitro cell growth. Consistent cell concentrations ensure reproducibility and accuracy in experiments. Cell counts are useful for tracking cell health and proliferation, determining immortalisation or transformation, seeding cells for...
LitStream

Chapter 10Cryopreservation of Cell Lines

Agarwal, Vinayak; Singh, Manisha

Pages: 147 –161

[Cryopreservation employs amalgamated applications of low temperatures in preserving structural composition along with functional integrity of animal cells and tissues. Traditionally, several cooling procedures permit ice crystal formation along with the rise in solute concentration that...

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