Open Advanced Search

Log in
Enterprise Plans
Sign Up

Log in
Sign Up
Enterprise Plans
Browse
Features
Pricing

DeepDyve

Advanced Search

show/hide query preview

With all of the words or DOIs:

DeepDyve's default query mode: search by keyword or DOI.

With the exact phrase:

Require these words, in this exact order. Do not surround your terms in double-quotes ("") in this field.

With at least one of the words:

Find any of these words, separated by spaces

Without the words:

Exclude each of these words, separated by spaces

With words in the article title:

Search for these terms only in the title of an article

Published between the years:

With the following authors:

Most effective as: LastName, First Name or Lastname, FN

With words in the journal name:

Search for articles published in journals where these words are in the journal name

Reset filters

Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

DeepDyve requires Javascript to function. Please enable Javascript on your browser to continue.

Advanced Technologies for Protein Complex Production and Characterization

Part of the Advances in Experimental Medicine and Biology Book Series (volume 896)

Editors: Vega, M. Cristina

DOI: 10.1007/978-3-319-27216-0

ISBN: 978-3-319-27214-6

Publisher: Springer International Publishing - Springer Journals

May 11, 2016

1 - 10 of 22 Chapters

LitStream

Protein Complex Production from the Drug Discovery Standpoint

Moarefi, Ismail

Pages: 3 –13

[Small molecule drug discovery critically depends on the availability of meaningful in vitro assays to guide medicinal chemistry programs that are aimed at optimizing drug potency and selectivity. As it becomes increasingly evident, most disease relevant drug targets do not act as a single...
LitStream

Choose a Suitable Expression Host: A Survey of Available Protein Production Platforms

Fernández, Francisco J.; Vega, M. Cristina

Pages: 15 –24

[Recombinant overexpression of a protein or a protein complex using any specific heterologous host can be an overwhelming challenge. The reasons may range from low yield and poor solubility of a single-subunit enzyme to the wrong stoichiometry or the incomplete assembly of a multiprotein complex....
LitStream

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts

Nie, Yan; Chaillet, Maxime; Becke, Christian; Haffke, Matthias; Pelosse, Martin; Fitzgerald, Daniel; Collinson, Ian; Schaffitzel, Christiane; Berger, Imre

Pages: 27 –42

[Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states. A multitude of applications in contemporary molecular and synthetic biology rely on efficient, robust and flexible methods to assemble...
LitStream

Complex Reconstitution and Characterization by Combining Co-expression Techniques in Escherichia coli with High-Throughput

Vincentelli, Renaud; Romier, Christophe

Pages: 43 –58

[Single protein expression technologies have strongly benefited from the Structural Genomics initiatives that have introduced parallelization at the laboratory level. Specifically, the developments made in the wake of these initiatives have revitalized the use of Escherichia coli as major host...
LitStream

Membrane Protein Production in E. coli for Applications in Drug Discovery

Snijder, Harm Jan (Arjan); Hakulinen, Jonna

Pages: 59 –77

[Producing high quality purified membrane proteins for structure-based drug design and biophysical assays compatible with typical timelines in drug discovery is a significant challenge. Escherichia coli has been an expression host of the utmost importance for soluble proteins and has applications...
LitStream

Cell-Free Synthesis of Macromolecular Complexes

Botte, Mathieu; Deniaud, Aurélien; Schaffitzel, Christiane

Pages: 79 –95

[Cell-free protein synthesis based on E. coli cell extracts has been described for the first time more than 50 years ago. To date, cell-free synthesis is widely used for the preparation of toxic proteins, for studies of the translation process and its regulation as well as for the incorporation...
LitStream

A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes

Biedendieck, Rebekka

Pages: 97 –113

[For many years the Gram-positive bacterium Bacillus megaterium has been used for the production and secretion of recombinant proteins. For this purpose it was systematically optimized. Plasmids with different inducible promoter systems, with different compatible origins, with small tags for...
LitStream

Protein Complex Production in Alternative Prokaryotic Hosts

Gómez, Sara; López-Estepa, Miguel; Fernández, Francisco J.; Vega, M. Cristina

Pages: 115 –133

[Research for multiprotein expression in nonconventional bacterial and archaeal expression systems aims to exploit particular properties of “alternative” prokaryotic hosts that might make them more efficient than E. coli for particular applications, especially in those areas where more...
LitStream

Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts

Fernández, Francisco J.; López-Estepa, Miguel; Querol-García, Javier; Vega, M. Cristina

Pages: 137 –153

[Protein complexes can be produced in multimilligram quantities using nonmethylotrophic and methylotrophic yeasts such as Saccharomyces cerevisiae and Komagataella (Pichia) pastoris. Yeasts have distinct advantages as hosts for recombinant protein production owing to their cost efficiency, ease...
LitStream

Leishmania tarentolae for the Production of Multi-subunit Complexes

Niimi, Tomoaki

Pages: 155 –165

[Multi-subunit protein complexes are involved in a wide variety of cellular processes including DNA replication, transcriptional regulation, signal transduction, protein folding and degradation. A better understanding of the function of these protein complexes requires structural insights into...

results per page

Browse All eBooks

Features

Search and discover articles on DeepDyve, PubMed, and Google Scholar
Read the full-text of open access and premium content
Organize articles with folders and bookmarks
Collaborate on and share articles and folders

Info

Pricing
Enterprise Plans
Browse Journals & Topics
About DeepDyve

Help

Help
Publishers
Contact Us

Sign up for your DeepDyve account now!

Read and print from thousands of top scholarly journals.

System error. Please try again!

Continue with Facebook

By signing up, you agree to DeepDyve’s Terms of Service and Privacy Policy.

Already have an account? Log in

Save Article to Bookmarks

Bookmark this article. You can see your Bookmarks on your DeepDyve Library.

To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.

Subscribe to Journal Email Alerts

To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don’t already have one.

Follow a Journal

To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one.

Our policy towards the use of cookies

All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.

cookie policy