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Multi-Parametric Live Cell Microscopy of 3D Tissue Models

Part of the Advances in Experimental Medicine and Biology Book Series (volume 1035)

Editors: Dmitriev, Ruslan I.

DOI: 10.1007/978-3-319-67358-5

ISBN: 978-3-319-67357-8

Publisher: Springer International Publishing - Springer Journals

Oct 28, 2017

1 - 10 of 12 Chapters

LitStream

Current State-of-the-Art 3D Tissue Models and Their Compatibility with Live Cell Imaging

Bardsley, Katie; Deegan, Anthony J.; El Haj, Alicia; Yang, Ying

Pages: 3 –18

[Mammalian cells grow within a complex three-dimensional (3D) microenvironment where multiple cells are organized and surrounded by extracellular matrix (ECM). The quantity and types of ECM components, alongside cell-to-cell and cell-to-matrix interactions dictate cellular differentiation,...
LitStream

Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation

Becker, Wolfgang; Shcheslavskiy, Vladislav; Rück, Angelika

Pages: 19 –30

[TCSPC FLIM/PLIM is based on a multi-dimensional time-correlated single-photon counting process. The sample is scanned by a high-frequency-pulsed laser beam which is additionally modulated on/off synchronously with the pixels of the scan. FLIM is obtained by building up the distribution of the...
LitStream

Quantitative Live Cell FLIM Imaging in Three Dimensions

Le Marois, Alix; Suhling, Klaus

Pages: 31 –48

[In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.]
LitStream

Three-Dimensional Tissue Models and Available Probes for Multi-Parametric Live Cell Microscopy: A Brief Overview

O’Donnell, Neil; Dmitriev, Ruslan I.

Pages: 49 –67

[In recent years, the advances in tissue engineering and regenerative medicine have resulted in introduction of novel 3D tissue models, materials and methods to the regular practice of cell biologists, material scientists and specialists from related areas. 3D tissue models allow mimicking in...
LitStream

Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues

Shpichka, Anastasia; Koroleva, Anastasia; Kuznetsova, Daria; Dmitriev, Ruslan I.; Timashev, Peter

Pages: 71 –81

[Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a...
LitStream

Multi-Parametric Imaging of Hypoxia and Cell Cycle in Intestinal Organoid Culture

Okkelman, Irina A.; Foley, Tara; Papkovsky, Dmitri B.; Dmitriev, Ruslan I.

Pages: 85 –103

[Dynamics of oxygenation of tissue and stem cell niches are important for understanding physiological function of the intestine in normal and diseased states. Only a few techniques allow live visualization of tissue hypoxia at cellular level and in three dimensions. We describe an optimized...
LitStream

Imaging of Intracellular pH in Tumor Spheroids Using Genetically Encoded Sensor SypHer2

Zagaynova, Elena V.; Druzhkova, Irina N.; Mishina, Natalia M.; Ignatova, Nadezhda I.; Dudenkova, Varvara V.; Shirmanova, Marina V.

Pages: 105 –119

[Intracellular pH (pHi) is one of the most important parameters that regulate the physiological state of cells and tissues. pHi homeostasis is crucial for normal cell functioning. Cancer cells are characterized by having a higher (neutral to slightly alkaline) pHi and lower (acidic) extracellular...
LitStream

Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments in a Single Cell

Nakabayashi, Takakazu; Awasthi, Kamlesh; Ohta, Nobuhiro

Pages: 121 –133

[Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images...
LitStream

Quantitative Imaging of Ca2+ by 3D–FLIM in Live Tissues

Rakymzhan, Asylkhan; Radbruch, Helena; Niesner, Raluca A.

Pages: 135 –141

[The calcium concentration within living cells is highly dynamic and, for many cell types, a reliable indicator of the functional state of the cells—both of isolated cells, but even, more important, of cells in tissue. In order to dynamically quantify intracellular calcium levels, various...
LitStream

Live Cell Imaging of Viscosity in 3D Tumour Cell Models

Shirmanova, Marina V.; Shimolina, Lubov’ E.; Lukina, Maria M.; Zagaynova, Elena V.; Kuimova, Marina K.

Pages: 143 –153

[Abnormal levels of viscosity in tissues and cells are known to be associated with disease and malfunction. While methods to measure bulk macroscopic viscosity of bio-tissues are well developed, imaging viscosity at the microscopic scale remains a challenge, especially in vivo. Molecular rotors...

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