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Essentials of Single-Cell Analysis

Part of the Series in BioEngineering Book Series

Editors: Tseng, Fan-Gang; Santra, Tuhin Subhra

DOI: 10.1007/978-3-662-49118-8

ISBN: 978-3-662-49116-4

Publisher: Springer Berlin Heidelberg - Springer Journals

Jan 22, 2016

1 - 10 of 15 Chapters

LitStream

Chapter 1Single-Cell Behavioral Assays for Heterogeneity Studies

Chen, Yu-Chih; Ingram, Patrick; Luan, Yi; Yoon, Euisik

Pages: 1 –29

[Cell heterogeneity is an emerging challenge in cell biology and cancer therapies. Each cell in heterogeneous populations has its own unique behavior, and thus responds differently to the same reagent or drug, making analysis or treatment difficult and complicated. Therefore, it is important to...
LitStream

Chapter 2Systems Biology in Single Cells

Glynn, Macdara; King, Damien; Ducrée, Jens

Pages: 31 –53

[From the beginning of the twenty-first century, there has been a shift towards studying biological processes using a holistic rather than a reductionist scientific paradigm thus establishing the approach now named “systems biology” or “systomics”. This method of biological investigation...
LitStream

Chapter 3Electroporation for Single-Cell Analysis

Santra, Tuhin Subhra; Tseng, Fan-Gang

Pages: 55 –83

[Single-cell analysis is a powerful technique to understand cell to cell or cell to environment behaviors. It can provide detailed information of cell proliferation, differentiation, and different responses to external stimuli and intracellular reactions. For single cell analysis, electroporation...
LitStream

Chapter 4Microinjection for Single-Cell Analysis

Shanmugam, Muniesh Muthaiyan; Santra, Tuhin Subhra

Pages: 85 –129

[The mere existence of life, from unicellular organisms to well-organized multicellular organisms, pathological conditions and death, has always fascinated human beings and demanded understanding of biological systems over several centuries. Further, an efficient treatment strategy for genetic...
LitStream

Chapter 5Optical Tools for Single-Cell Manipulation and Analysis

Casey, Duncan; Dooley, Jayne

Pages: 131 –157

[Experiments on individual cells require a range of extremely precise tools to permit their selection, manipulation, stimulation and analysis. This is further complicated by the cells’ sensitivity to their environment, meaning that such tools must also be very gentle (or at least very localised)...
LitStream

Chapter 6Optoelectrokinetic Manipulation for Cell Analysis

Chuang, Han-Sheng; Ku, Hu-Yao; Li, Fu-Tsun; Kumar, Aloke; Wang, Jhih-Cheng; Wang, Kuan-Chih

Pages: 159 –194

[Rapid advancement of microfluidic technology in the recent years has opened a new era for biological diagnostics. The demands for fast response, multi-functionality, non-invasiveness, and programmability boost the developments in many fields, such as tissue engineering, cell analysis, and...
LitStream

Chapter 7Continuous Micro-/Nanofluidic Devices for Single-Cell Analysis

Chen, Chihchen

Pages: 195 –211

[Micro- and nanofluidic devices are revolutionizing the fields of single-cell analysis, and benefiting related efforts in life science research, agricultural industry, and clinical medicine. These miniaturized devices introduce much desired capabilities in accurate cell and fluid handling, and...
LitStream

Chapter 8Single-Cell Mechanical Properties: Label-Free Biomarkers for Cell Status Evaluation

Chen, Jian; Huang, Song-Bin; Xue, Chengcheng; Fan, Beiyuan; Chen, Deyong; Wang, Junbo; Wu, Min-Hsien

Pages: 213 –234

[The mechanical behavior of biological cells is largely determined by their cytoskeletons; abnormal cellular functions can change cytoskeletons, leading to variations in cellular mechanical properties. This chapter begins with a summary of the relationships between cellular mechanical properties...
LitStream

Chapter 9Cytometry of Single-Cells for Biology and Biomedicine

Leary, James F.

Pages: 235 –255

[Flow and image cytometry are mature single-cell analysis technologies that have been developed over the past 50 years. Fluorescence has been the primary mode of generating single-cell signals. There are now hundreds of different color fluorescent probes linked to thousands of different molecules...
LitStream

Chapter 10Single-Cell Genomics and Epigenomics

Valdés-Mora, Fátima; Lee, Heather J.

Pages: 257 –301

[The cell is the fundamental unit of life, in which the blueprint of the genome is transcribed and translated into biological function. Most of our current understanding of the genome, epigenome, and transcriptome is based on analysis of millions of cells as a bulk population. These analyses are...

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