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Ultrashort Electric Pulse Effects in Biology and Medicine

Part of the Series in BioEngineering Book Series

DOI: 10.1007/978-981-10-5113-5

ISBN: 978-981-10-5112-8

Publisher: Springer Singapore - Springer Journals

May 22, 2021

1 - 10 of 19 Chapters

LitStream

Chapter 1Introduction

Schoenbach, Karl H.

Pages: 1 –31

[The effects of intense electrical pulses on cell membranes have been studied extensively since the second half of the twentieth century, and have found multiple applications, ranging from bacterial decontamination to medical therapies. The first studies on this effect, named electroporation,...
LitStream

Chapter 2Effects of usEPs on Plasma Membranes—Pores, Channels, and Repair

Beebe, Stephen J.

Pages: 33 –75

[Receptors, channels, glycoprotein, and transport proteins, among other integral proteins, load the plasma membrane lipid bilayer, which receives reinforcement from cytoskeletal filaments as the boundaries of cellular life. Early studies suggested that usEPs did not induce plasma membrane...
LitStream

Chapter 3Simulations of Membrane Effects of Cells After Exposure to Ultrashort Pulses

Joshi, Ravi

Pages: 77 –108

[Externally applied nanosecond electric pulses are useful to trigger and tailor bioeffects in cells and tissues. However, the parameter space is large given the different types of cells, and the wide range of potential electrical parameters (such as pulse durations and waveforms, field...
LitStream

Chapter 4Comparison Between Monopolar and Bipolar Pulses for Effective Nanoporation

Joshi, Ravi

Pages: 109 –125

[The utility of electric pulsing for attaining and driving a range of bio-effects have already been discussed. This chapter focuses and probes the role of pulse shape (e.g., monopolar-vs.-bipolar), multiple electrode scenarios, and serial-versus-simultaneous pulsing. A possible analysis based on...
LitStream

Chapter 5usEP Effects on the Endoplasmic Reticulum (ER)

Beebe, Stephen J.

Pages: 127 –141

[The endoplasmic reticulum (ER) is a tubular membranous labyrinth throughout the cytoplasm that is continuous with the nuclear membrane, exhibits contact sites with mitochondria and the plasma membrane, and displays domains for specific functions. It is smooth or rough with ribosomes for protein...
LitStream

Chapter 6Intra-cellular Calcium Release Dynamics Due to Nanosecond Electric Pulsing

Joshi, Ravi

Pages: 143 –157

[Permeabilization of cell membranous structures by nanosecond electric field pulses triggers a transient rise of cytosolic calcium with multifarious downstream effects. Electroporation of intracellular membranes (such as those of the Endoplasmic Reticulum) are likely responsible for the calcium...
LitStream

Chapter 7Effects of usEPs on DNA, Nuclear, and Subnuclear Compartments

Beebe, Stephen J.

Pages: 159 –174

[As the largest intracellular structure in mammalian cells, the nucleus, its double phospholipid nuclear envelope, and its chromatin/DNA content were suspected targets for usEPs. Many different methods were used to determine DNA/ nuclear damage including analyses with the comet assay, DNA...
LitStream

Chapter 8Mitochondria as usEP Sensors

Beebe, Stephen J.

Pages: 175 –226

[The endocytotic and symbiotic inclusion of a prokaryote by an early eukaryote, its subsequent evolution as mitochondria, and its collaboration with the nucleus provided these new symbiotes with enough ATP to evolve a new world of extraordinarily diverse organisms. Mitochondria assumed roles for...
LitStream

Chapter 9usEP Induce Regulated Cell Death Mechanisms

Beebe, Stephen J.

Pages: 227 –263

[Cells employs many different mechanisms for their expiration. The best known and studied regulated cell death mechanism is apoptosis. Cell death subtypes, including regulated cell death (RCD), programmed cell death (PCD), and accidental cell death, are discussed. A discussion on and caution for...
LitStream

Chapter 10Model Rate Equation Evaluation of an Extrinsic Apoptotic Pathway

Joshi, Ravi

Pages: 265 –274

[Apoptosis is one of the most complex signaling pathways, and can be triggered by a number of factors, radiation, chemotherapeutic drugs, electric field application, etc. The apoptotic pathways can generally be divided into signaling via the death receptors (extrinsic pathway) or the mitochondria...

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