Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Micropropagation of Bunium Persicum (Boiss.) B. Fedtsch. via Indirect Somatic Embryogenesis

Micropropagation of Bunium Persicum (Boiss.) B. Fedtsch. via Indirect Somatic Embryogenesis Bunium persicum (Boiss.) B. Fedtsch., a member of the Apiaceae family, is one of the oldest and most economically important species. Because of seed dormancy and lack of hypocotyl growth when cotyledon emerged, In vivo cultivation of Bunium persicum has not succeeded. In order to investigate the effects of some plant growth regulators on somatic embryogenesis of Bunium persicum in tissue cultured condition, we have used indirect embryogenesis technique. Explant harvested from root, hypocotyl and cotyledon (leaf) were investigated on MS media combined with 2, 4-D, BAP, NAA (each of those with 6 concentration levels: 0, 0.5, 1, 2, 3 and 4 mg/l) and furthermore Kin (using 3 concentration levels: 0, 0.2 and 0.5 mg/l). Calli developed into different media and after 3 or 4 weeks some of them regenerated embryos. The best indirect somatic embryogenesis was obtained in a medium treated with root explants and the highest indirect embryogenesis was resulted from media treated with 2 mg/l 2, 4-D, 0.5 mg/l BAP or 1 mg/l BAP + 0.2 Kin. Also the highest percentage of callogenesis was observed on MS media containing 0.5 mg/l NAA+0.5 mg/l Kin, 4mg/l NAA or 2 mg/l 2, 4-D + 0.5 mg/l Kin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Agricultural Engineering and Biotechnology Bowen Publishing Company Ltd.

Micropropagation of Bunium Persicum (Boiss.) B. Fedtsch. via Indirect Somatic Embryogenesis

Download PDF
6 pages

Loading next page...
 
/lp/bowen-publishing-company-ltd/micropropagation-of-bunium-persicum-boiss-b-fedtsch-via-indirect-0B2xWwqYjg

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Bowen Publishing Company Ltd.
Copyright
Copyright © BOWEN PUBLISHING COMPANY LTD.
ISSN
2331-3234
eISSN
2331-3463
DOI
10.18005/JAEB0103001
Publisher site
See Article on Publisher Site

Abstract

Bunium persicum (Boiss.) B. Fedtsch., a member of the Apiaceae family, is one of the oldest and most economically important species. Because of seed dormancy and lack of hypocotyl growth when cotyledon emerged, In vivo cultivation of Bunium persicum has not succeeded. In order to investigate the effects of some plant growth regulators on somatic embryogenesis of Bunium persicum in tissue cultured condition, we have used indirect embryogenesis technique. Explant harvested from root, hypocotyl and cotyledon (leaf) were investigated on MS media combined with 2, 4-D, BAP, NAA (each of those with 6 concentration levels: 0, 0.5, 1, 2, 3 and 4 mg/l) and furthermore Kin (using 3 concentration levels: 0, 0.2 and 0.5 mg/l). Calli developed into different media and after 3 or 4 weeks some of them regenerated embryos. The best indirect somatic embryogenesis was obtained in a medium treated with root explants and the highest indirect embryogenesis was resulted from media treated with 2 mg/l 2, 4-D, 0.5 mg/l BAP or 1 mg/l BAP + 0.2 Kin. Also the highest percentage of callogenesis was observed on MS media containing 0.5 mg/l NAA+0.5 mg/l Kin, 4mg/l NAA or 2 mg/l 2, 4-D + 0.5 mg/l Kin.

Journal

Journal of Agricultural Engineering and Biotechnology – Bowen Publishing Company Ltd.

Published: Nov 21, 2013

There are no references for this article.