Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Wells, O. Awa (1970)
New technique for assessing acrosomal characteristics of spermatozoa.Journal of dairy science, 53 2
R. Saacke (1972)
Semen quality tests and their relationship to fertility
P. Watson, I. Martin (1972)
A comparison of changes in the acrosomes of deep-frozen ram and bull spermatozoa.Journal of reproduction and fertility, 28 1
D. Pontbriand, J. Howard, M. Schiewe, L. Stuart, D. Wildt (1989)
Effect of cryoprotective diluent and method of freeze-thawing on survival and acrosomal integrity of ram spermatozoa.Cryobiology, 26 4
Tetsuya Kodai, K. Umebayashi, T. Nakatani, K. Ishiyama, N. Noda (2007)
Compositions of royal jelly II. Organic acid glycosides and sterols of the royal jelly of honeybees (Apis mellifera).Chemical & pharmaceutical bulletin, 55 10
Juan Alvarez, Bayard Storey (1989)
Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation.Gamete research, 23 1
R. Vishwanath, P. Shannon (1997)
Do sperm cells age? A review of the physiological changes in sperm during storage at ambient temperature.Reproduction, fertility, and development, 9 3
P. Shannon, B. Curson (1972)
Toxic effect and action of dead sperm on diluted bovine semen.Journal of dairy science, 55 5
M. Gancarczyk, Magda Kuklińska, J. Sadowska, J. Strzeżek, B. Bilińska (2006)
Aromatization and antioxidant capacity in the testis of seasonally breeding bank voles: Effects of LH, PRL and IGF-I.Theriogenology, 65 7
D. Garner, Cheryl Thomas, Hannes Joerg, J. Dejarnette, C. Marshall (1997)
Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.Biology of reproduction, 57 6
J. Cummins, A. Jequier, Raymond Kan (1994)
Molecular biology of human male infertility: Links with aging, mitochondrial genetics, and oxidative stress?Molecular Reproduction and Development, 37
P. Fišer, C. Hansen, L. Underhill, G. Marcus (1991)
New thermal stress test to assess the viability of cryopreserved boar sperm.Cryobiology, 28 5
H. Rodríguez-Martínez (2000)
Evaluación de semen congelado. Métodos tradicionales y de actualidadInt. Vet. Inf. Serv
Aitken Rj (1994)
Pathophysiology of human spermatozoa.Current Opinion in Obstetrics & Gynecology, 6
Inge Ebisch, Frank Pierik, F. Jong, C. Thomas, R. Steegers-Theunissen (2006)
Does folic acid and zinc sulphate intervention affect endocrine parameters and sperm characteristics in men?International journal of andrology, 29 2
S. Biswas, K. Ferguson, J. Stedronska, G. Baffoe, M. Mansfield, M. Kosbab (1978)
Fructose and hormone levels in semen: their correlations with sperm counts and motility.Fertility and sterility, 30 2
P. Guérin, S. Mouatassim, Y. Menezo (2001)
Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings.Human reproduction update, 7 2
S. Howe, P. Dimick, A. Benton (1985)
Composition of freshly harvested and commercial royal jellyJournal of Apicultural Research, 24
J. Krzyżosiak, P. Molan, R. Vishwanath (1999)
Measurements of bovine sperm velocities under true anaerobic and aerobic conditions.Animal reproduction science, 55 3-4
Eve De, Lamirande And, C. Gagnon
Reactive Oxygen Species and Human Spermatozoa I. Effects on the Motility of Intact Spermatozoa and on Sperm Axonemes
Ameliorating effect of royal jelly on viability and longevity of frozen-thawed buffalo spermatozoa Cryopreserved buffalo semen is generally acknowledged to have an impaired fertility by comparison with fresh semen. The reduction arises from both a lower viability post-thaw and sublethal dysfunction in a proportion of the surviving subpopulation. Thus, the present study was conducted to observe the effect of addition of royal jelly on subsequent survival and acrosomal integrity of buffalo spermatozoa during post-thawing incubation. Frozen-thawed semen samples were washed in Tris extender containing different concentrations of royal jelly (RJ): 0.0 control, 0.1, 0.2, 0.3, 0.4, 0.5%. Spermatozoa motility and viability in addition acrosomal integrity of thawed samples were assessed after thawing and at 30 min intervals during incubation at 37°C for 120 min. Results of experiment showed that the percentages of sperm function parameters were improved with an increase of royal jelly in Tris extender from 0.0 to 0.4% (P < 0.05). Statistical analysis for sperm motility and viability in addition acrosomal integrity (Tables 1, 2, 3) showed that best results (significant higher P < 0.05) occurred with RJ doses of 0.4 and 0.5% especially at 30-60 min incubation periods than that control. Therefore, the addition of 0.4% of royal jelly in Tris extender is recommended for dilution of thawed semen. These results indicated that addition of 0.4% RJ to Tris-buffer enhance and maintain the viability and longevity of buffalo spermatozoa for a long period and this additive can be used for increasing the possibility of collision between spermatozoa and ova during insemination.
Annales UMCS, Medicina Veterinaria – de Gruyter
Published: Jan 1, 2010
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.