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70 Inhalation Exposure of Copper Particles - Quantitative Differentiation Between Copper Oxides and Metallic Copper

70 Inhalation Exposure of Copper Particles - Quantitative Differentiation Between Copper Oxides... Abstracts i93 pristine 200nm silica nanoparticles. Following 6 to 48 hours cell viability, but an increased DNA damage could be detected of exposure, mitochondrial activity and viability were found in epithelial cells. All in all, UFP with low organic content seem to be significantly reduced when exposed to SAD particles to induce a stronger acute cytotoxic and oxidative stress re- at concentrations 2.5µg/mL or higher. Oxygen consumption sponse in epithelial cells, which might cause secondary effects rate (OCR) and pH were investigated with a Seahorse XF in fibroblasts. Analyzer, revealing significant changes to cellular metabolism across treatments as early as 6 hours following exposure. While most treatments slightly increased respiration, SAD Abstract citation ID: wxac087.228 SiNPs were found to inhibit mitochondrial function, reduce ATP generation, increase reliance on glycolysis, and reduce 70 Inhalation Exposure of Copper glycolytic reserve. Metabolomic analysis following 24 hours Particles - Quantitative Differentiation of exposure found several cellular energetics pathways (e.g., Between Copper Oxides and Metallic fatty acid metabolism, glycolysis, and TCA cycle) significantly altered across ash-based treatments. Such changes indicate Copper that exposure to sugarcane ash and its derivatives can pro- Katharina  Bluemlein, Thorben  Schirmer, Oliver  Licht, mote mitochondrial dysfunction and disrupt metabolic ac- Sven Schuchardt Fraunhofer ITEM, Hannover, Germany tivity of human PCT cells. Inhalation exposure to copper and its inorganic compounds can occur in various industrial sectors such as ore mining, production of electrical equipment etc. Toxic effects of copper Abstract citation ID: wxac087.227 and its oxides have been shown to be attributed to i. particle size and ii. dissolution/release of copper ions. The latter being 68 On the Role of Chemical Identity dependend on the chemical composition of the copper particle over the size of Model Ultrafine Particles and its size. Hence, in the context of occupational inhalation Driven (geno)-Toxicity exposure to those compounds emphasis should not only be dir- ected towards particle size but also to the copper species. We 1,2 1,2 1,2 Jana Pantzke , Anusmita Das , Elias J. Zimmermann , have developed an ICP-MS based method that allows quanti- 1,2 1,2 1 Svenja Offer , Seongho Jeong , Mathilde N. Delaval , tative distinction between copper oxides (Cu O) and metallic 1 1 Jürgen Schnelle-Kreis , Martin Sklorz , Sebastiano Di Buc copper (mCu) upon sampling on mixed-cellulose ester (MCE) 1,2 1,2 1 chianico , Ralf  Zimmermann Helmholtz Zentrum membrane filters. This approach is based on the different dis - München, Munich, Germany. University of Rostock, solution properties of Cu O and mCu in dilute acid. Systematic Rostock, Germany assessment of the approach in regard to filter load and extrac - Especially through the release from anthropogenic sources, tion time have been conducted and successfully applied to field there is growing concern about human exposure to ultrafine samples. particulate matter (UFP). Inhalation of UFP is associated with respiratory diseases such as asthma, pulmonary fibrosis and lung cancer, but their mechanisms of action are still vastly Abstract citation ID: wxac087.229 unknown. In this study we aimed to address the question whether the physical characteristics of UFP contribute most to 74 Impact of Air Pollution on Lung their toxicity or whether the particles primarily act as carriers Immune Responses in a Mouse Model of of toxic chemicals. For this scope, two distinct UFP aerosols Influenza Infection with low or high content of semi-volatile organic compounds 1 1 2,3 (SVOC) but comparable sizes were produced by a combustion Lydie  Martín-Fai vre , Lisa  Prince , Chloé  Chi vé , 1 2 aerosol standard soot generator and investigated regarding Jean-Michel  Sallenave , Armelle  Baeza-Squiban , 1 1 their acute (geno)-toxic potential in a cell model consisting of Ignacio  Garcia-Verdugo Laboratoire d’excellence alveolar epithelial cells, macrophages, and fibroblasts at the air- Inflamex, UMR1152-INSERM, Université Paris Cité, Paris, liquid interface. Low SVOC UFP induced an increased cytotox- France. Unité de biologie fonctionnelle et adaptative, icity accompanied by a reduced cell number of epithelial cells UMR8251 CNRS, Université Paris Cité, Paris, France. and a decreased metabolic activity of fibroblasts. Furthermore, Agence de l’Environnement et de la Maîtrise de l’Energie, an increased intracellular release of reactive oxygen species 20 avenue du Grésillé- BP 90406 49004 Angers Cedex 01, (ROS) was observed after low SVOC UFP treatment, which re- Angers, France sulted in a depletion of intracellular glutathione. The increased During air pollution peaks, influenza infections are more se - ROS release could be causative for the observed secondary vere, and the number of hospitalizations associated to flu in - genotoxicity in fibroblasts exclusively detected after exposure creases. This project aims to study if air pollution contributes to low organic content UFP. High SVOC UFP did not impact http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Work Exposures and Health Oxford University Press

70 Inhalation Exposure of Copper Particles - Quantitative Differentiation Between Copper Oxides and Metallic Copper

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Publisher
Oxford University Press
Copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.
ISSN
2398-7308
eISSN
2398-7316
DOI
10.1093/annweh/wxac087.228
Publisher site
See Article on Publisher Site

Abstract

Abstracts i93 pristine 200nm silica nanoparticles. Following 6 to 48 hours cell viability, but an increased DNA damage could be detected of exposure, mitochondrial activity and viability were found in epithelial cells. All in all, UFP with low organic content seem to be significantly reduced when exposed to SAD particles to induce a stronger acute cytotoxic and oxidative stress re- at concentrations 2.5µg/mL or higher. Oxygen consumption sponse in epithelial cells, which might cause secondary effects rate (OCR) and pH were investigated with a Seahorse XF in fibroblasts. Analyzer, revealing significant changes to cellular metabolism across treatments as early as 6 hours following exposure. While most treatments slightly increased respiration, SAD Abstract citation ID: wxac087.228 SiNPs were found to inhibit mitochondrial function, reduce ATP generation, increase reliance on glycolysis, and reduce 70 Inhalation Exposure of Copper glycolytic reserve. Metabolomic analysis following 24 hours Particles - Quantitative Differentiation of exposure found several cellular energetics pathways (e.g., Between Copper Oxides and Metallic fatty acid metabolism, glycolysis, and TCA cycle) significantly altered across ash-based treatments. Such changes indicate Copper that exposure to sugarcane ash and its derivatives can pro- Katharina  Bluemlein, Thorben  Schirmer, Oliver  Licht, mote mitochondrial dysfunction and disrupt metabolic ac- Sven Schuchardt Fraunhofer ITEM, Hannover, Germany tivity of human PCT cells. Inhalation exposure to copper and its inorganic compounds can occur in various industrial sectors such as ore mining, production of electrical equipment etc. Toxic effects of copper Abstract citation ID: wxac087.227 and its oxides have been shown to be attributed to i. particle size and ii. dissolution/release of copper ions. The latter being 68 On the Role of Chemical Identity dependend on the chemical composition of the copper particle over the size of Model Ultrafine Particles and its size. Hence, in the context of occupational inhalation Driven (geno)-Toxicity exposure to those compounds emphasis should not only be dir- ected towards particle size but also to the copper species. We 1,2 1,2 1,2 Jana Pantzke , Anusmita Das , Elias J. Zimmermann , have developed an ICP-MS based method that allows quanti- 1,2 1,2 1 Svenja Offer , Seongho Jeong , Mathilde N. Delaval , tative distinction between copper oxides (Cu O) and metallic 1 1 Jürgen Schnelle-Kreis , Martin Sklorz , Sebastiano Di Buc copper (mCu) upon sampling on mixed-cellulose ester (MCE) 1,2 1,2 1 chianico , Ralf  Zimmermann Helmholtz Zentrum membrane filters. This approach is based on the different dis - München, Munich, Germany. University of Rostock, solution properties of Cu O and mCu in dilute acid. Systematic Rostock, Germany assessment of the approach in regard to filter load and extrac - Especially through the release from anthropogenic sources, tion time have been conducted and successfully applied to field there is growing concern about human exposure to ultrafine samples. particulate matter (UFP). Inhalation of UFP is associated with respiratory diseases such as asthma, pulmonary fibrosis and lung cancer, but their mechanisms of action are still vastly Abstract citation ID: wxac087.229 unknown. In this study we aimed to address the question whether the physical characteristics of UFP contribute most to 74 Impact of Air Pollution on Lung their toxicity or whether the particles primarily act as carriers Immune Responses in a Mouse Model of of toxic chemicals. For this scope, two distinct UFP aerosols Influenza Infection with low or high content of semi-volatile organic compounds 1 1 2,3 (SVOC) but comparable sizes were produced by a combustion Lydie  Martín-Fai vre , Lisa  Prince , Chloé  Chi vé , 1 2 aerosol standard soot generator and investigated regarding Jean-Michel  Sallenave , Armelle  Baeza-Squiban , 1 1 their acute (geno)-toxic potential in a cell model consisting of Ignacio  Garcia-Verdugo Laboratoire d’excellence alveolar epithelial cells, macrophages, and fibroblasts at the air- Inflamex, UMR1152-INSERM, Université Paris Cité, Paris, liquid interface. Low SVOC UFP induced an increased cytotox- France. Unité de biologie fonctionnelle et adaptative, icity accompanied by a reduced cell number of epithelial cells UMR8251 CNRS, Université Paris Cité, Paris, France. and a decreased metabolic activity of fibroblasts. Furthermore, Agence de l’Environnement et de la Maîtrise de l’Energie, an increased intracellular release of reactive oxygen species 20 avenue du Grésillé- BP 90406 49004 Angers Cedex 01, (ROS) was observed after low SVOC UFP treatment, which re- Angers, France sulted in a depletion of intracellular glutathione. The increased During air pollution peaks, influenza infections are more se - ROS release could be causative for the observed secondary vere, and the number of hospitalizations associated to flu in - genotoxicity in fibroblasts exclusively detected after exposure creases. This project aims to study if air pollution contributes to low organic content UFP. High SVOC UFP did not impact

Journal

Annals of Work Exposures and HealthOxford University Press

Published: May 10, 2023

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