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Characterization of the N-linked glycans of Giardia intestinalis

Characterization of the N-linked glycans of Giardia intestinalis This article reports the first rigorous evidence for the existence of N-glycans in Giardia intestinalis, a parasite that is a widespread human pathogen, being a major cause of enteric disease in the world. Excreted/secreted molecules of G. intestinalis are known to stimulate the immune system. Structural strategies based on MALDI and electrospray mass spectrometry were employed to examine the excreted/secreted molecules for their N-glycan content. These revealed that the major oligosaccharides released by peptide N-glycosidase F are complex-type structures and correspond to bi-, and triantennary structures without core (α1,6) fucosylation. The major nonreducing epitopes in these complex-type glycans are: Galβ1-4GlcNAc (LacNAc) and NeuAc α2-6Galβ1-4GlcNAc (sialylated LacNAc). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glycobiology Oxford University Press

Characterization of the N-linked glycans of Giardia intestinalis

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References (48)

Publisher
Oxford University Press
Copyright
Glycobiology vol. 15 no. 5 © Oxford University Press 2004; all rights reserved.
ISSN
0959-6658
eISSN
1460-2423
DOI
10.1093/glycob/cwi035
pmid
15616121
Publisher site
See Article on Publisher Site

Abstract

This article reports the first rigorous evidence for the existence of N-glycans in Giardia intestinalis, a parasite that is a widespread human pathogen, being a major cause of enteric disease in the world. Excreted/secreted molecules of G. intestinalis are known to stimulate the immune system. Structural strategies based on MALDI and electrospray mass spectrometry were employed to examine the excreted/secreted molecules for their N-glycan content. These revealed that the major oligosaccharides released by peptide N-glycosidase F are complex-type structures and correspond to bi-, and triantennary structures without core (α1,6) fucosylation. The major nonreducing epitopes in these complex-type glycans are: Galβ1-4GlcNAc (LacNAc) and NeuAc α2-6Galβ1-4GlcNAc (sialylated LacNAc).

Journal

GlycobiologyOxford University Press

Published: May 22, 2005

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