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Evaluation and control of Alternaria alternata causing leaf spot in soybean in Northeast China

Evaluation and control of Alternaria alternata causing leaf spot in soybean in Northeast China Abstract Aim The aims of this study were to characterize the causal agent of soybean leaf spot and determine the pathogenicity of the pathogen to the main crops in Northeast China and detect the sensitivity of pathogens to the main chemical fungicides and the potted control effect. Methods and results In 2020 and 2021, an uncommon leaf spot was observed in Harbin, Heilongjiang Province (125°42′-130°10′E, 44°04′-46°40′N), China. The pathogen can infect soybean leaves and cause leaf spot. We collected diseased soybean leaves and isolated four pathogen organisms, all of which were identified as Alternaria alternata through Morphological and Molecular Identification. Koch's postulates were used to confirm pathogenicity. To the best of our knowledge, this is the first report on soybean leaf spot disease caused by A. alternata in northeast China. Moreover, A. alternata had a broad host range and caused leaf spot in most legumes. However, it did not infect medicated lentil (Dolicho Lablab L.) or tobacco (Nicotiana tabacum L.). Assessment of A. alternaria susceptibility to fungicides by spore germination method, isolates of A. alternata were most sensitive to flusilazole, with EC50 values of 0.0040 - 0.0053 μg ml−1. Through two pot experiments, the average control efficacy of 0.1 mg ml−1 flusilazole on soybean leaf spot caused by A. alternata was 80.7%. Conclusions The work reported that A. alternata is the pathogen organism that causes soybean leaf spot in northern China. The pathogen organism can infect a variety of leguminous plants. Considering the control cost and effect, flusilazole is more suitable for controlling leaf spot disease in the field, and benzoxystrobin can be used as an alternative fungicide. Alternaria alternata, fungicide efficacy, Glycine max, host range, identification, leaf spot Accepted manuscripts Accepted manuscripts are PDF versions of the author’s final manuscript, as accepted for publication by the journal but prior to copyediting or typesetting. They can be cited using the author(s), article title, journal title, year of online publication, and DOI. They will be replaced by the final typeset articles, which may therefore contain changes. The DOI will remain the same throughout. PDF This content is only available as a PDF. © The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Oxford University Press

Evaluation and control of Alternaria alternata causing leaf spot in soybean in Northeast China

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Copyright
Copyright © 2023 Society for Applied Microbiology
ISSN
0021-8847
eISSN
1365-2672
DOI
10.1093/jambio/lxad004
Publisher site
See Article on Publisher Site

Abstract

Abstract Aim The aims of this study were to characterize the causal agent of soybean leaf spot and determine the pathogenicity of the pathogen to the main crops in Northeast China and detect the sensitivity of pathogens to the main chemical fungicides and the potted control effect. Methods and results In 2020 and 2021, an uncommon leaf spot was observed in Harbin, Heilongjiang Province (125°42′-130°10′E, 44°04′-46°40′N), China. The pathogen can infect soybean leaves and cause leaf spot. We collected diseased soybean leaves and isolated four pathogen organisms, all of which were identified as Alternaria alternata through Morphological and Molecular Identification. Koch's postulates were used to confirm pathogenicity. To the best of our knowledge, this is the first report on soybean leaf spot disease caused by A. alternata in northeast China. Moreover, A. alternata had a broad host range and caused leaf spot in most legumes. However, it did not infect medicated lentil (Dolicho Lablab L.) or tobacco (Nicotiana tabacum L.). Assessment of A. alternaria susceptibility to fungicides by spore germination method, isolates of A. alternata were most sensitive to flusilazole, with EC50 values of 0.0040 - 0.0053 μg ml−1. Through two pot experiments, the average control efficacy of 0.1 mg ml−1 flusilazole on soybean leaf spot caused by A. alternata was 80.7%. Conclusions The work reported that A. alternata is the pathogen organism that causes soybean leaf spot in northern China. The pathogen organism can infect a variety of leguminous plants. Considering the control cost and effect, flusilazole is more suitable for controlling leaf spot disease in the field, and benzoxystrobin can be used as an alternative fungicide. Alternaria alternata, fungicide efficacy, Glycine max, host range, identification, leaf spot Accepted manuscripts Accepted manuscripts are PDF versions of the author’s final manuscript, as accepted for publication by the journal but prior to copyediting or typesetting. They can be cited using the author(s), article title, journal title, year of online publication, and DOI. They will be replaced by the final typeset articles, which may therefore contain changes. The DOI will remain the same throughout. PDF This content is only available as a PDF. © The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Journal of Applied MicrobiologyOxford University Press

Published: Jan 11, 2023

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