Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of carbohydrate chains.

On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of... One method used to determine the primary sequence of oligosaccharides is to digest them with exoglycosidases and analyze the resulting digestion products by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Previous research has demonstrated that these digestions can be performed on the MALDI target. However, the procedure requires the sample to be incubated at elevated temperatures, and complete digestion requires a few hours. We demonstrate new conditions that permit exoglycosidase digestions to be performed on the MALDI target at room temperature within 30 min. Oligosaccharide standards were digested with one or more exoglycosidases to show that the enzymes retain their activity and specificity under these new reaction conditions. Using this method, the primary sequences of carbohydrate chains can be determined in a relatively short amount of time. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Pubmed

On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of carbohydrate chains.

Analytical Chemistry , Volume 71 (7): -1396 – Jun 1, 1999

On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of carbohydrate chains.


Abstract

One method used to determine the primary sequence of oligosaccharides is to digest them with exoglycosidases and analyze the resulting digestion products by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Previous research has demonstrated that these digestions can be performed on the MALDI target. However, the procedure requires the sample to be incubated at elevated temperatures, and complete digestion requires a few hours. We demonstrate new conditions that permit exoglycosidase digestions to be performed on the MALDI target at room temperature within 30 min. Oligosaccharide standards were digested with one or more exoglycosidases to show that the enzymes retain their activity and specificity under these new reaction conditions. Using this method, the primary sequences of carbohydrate chains can be determined in a relatively short amount of time.

Loading next page...
 
/lp/pubmed/on-target-exoglycosidase-digestions-maldi-ms-for-determining-the-j9aWuDdhSG

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

ISSN
0003-2700
DOI
10.1021/ac980980u
pmid
10204046

Abstract

One method used to determine the primary sequence of oligosaccharides is to digest them with exoglycosidases and analyze the resulting digestion products by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Previous research has demonstrated that these digestions can be performed on the MALDI target. However, the procedure requires the sample to be incubated at elevated temperatures, and complete digestion requires a few hours. We demonstrate new conditions that permit exoglycosidase digestions to be performed on the MALDI target at room temperature within 30 min. Oligosaccharide standards were digested with one or more exoglycosidases to show that the enzymes retain their activity and specificity under these new reaction conditions. Using this method, the primary sequences of carbohydrate chains can be determined in a relatively short amount of time.

Journal

Analytical ChemistryPubmed

Published: Jun 1, 1999

There are no references for this article.