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3D Electro-Rotation of Single CellsOpto-Electronic Integration of Thick-Electrode DEP Microfluidic Chip

3D Electro-Rotation of Single Cells: Opto-Electronic Integration of Thick-Electrode DEP... CHAPTER 3 Opto-Electronic Integration of Thick- Electrode DEP Microfluidic Chip 3.1 INTRODUCTION The multi-electrode structure of thick-electrode DEP overcomes the limitation of electric field attenuation and small working range of the 2D planar electrodes, and enables single-cell 3D rota- tion. With the development of MEMS technology, the thick-electrode structure can be more easily combined with other technologies to realize multi-function. In order to expand the functions of the multi-electrode structure of thick-electrode DEP, this chapter redesigns the single-cell 3D rotation chip structure, integrates dual-beam optical stretcher into the chip, and realizes multi-parameter measurement of single cells. Single-cell multi-parameter measurement Single cells are highly heterogeneous and can be distinguished by cell markers [172, 173]. Cell markers refer to biochemical indicators that can objectively measure and evaluate cells [174, 175]. Examination of cell markers with certain specificity plays an important role in the identification, early diagnosis and prevention of diseases, and therapeutic monitoring. At present, the measure- ment and characterization of cell markers has gradually become an important hot spot in research. Cell markers are classified into extrinsic biochemical markers [ 176] and intrinsic markers [177]. The status of cells can be identified by external biochemical markers (e.g., fluorescent pigments, quantum http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

3D Electro-Rotation of Single CellsOpto-Electronic Integration of Thick-Electrode DEP Microfluidic Chip

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Publisher
Springer International Publishing
Copyright
© Springer Nature Switzerland AG 2020
ISBN
978-3-031-00538-1
Pages
57 –78
DOI
10.1007/978-3-031-01666-0_3
Publisher site
See Chapter on Publisher Site

Abstract

CHAPTER 3 Opto-Electronic Integration of Thick- Electrode DEP Microfluidic Chip 3.1 INTRODUCTION The multi-electrode structure of thick-electrode DEP overcomes the limitation of electric field attenuation and small working range of the 2D planar electrodes, and enables single-cell 3D rota- tion. With the development of MEMS technology, the thick-electrode structure can be more easily combined with other technologies to realize multi-function. In order to expand the functions of the multi-electrode structure of thick-electrode DEP, this chapter redesigns the single-cell 3D rotation chip structure, integrates dual-beam optical stretcher into the chip, and realizes multi-parameter measurement of single cells. Single-cell multi-parameter measurement Single cells are highly heterogeneous and can be distinguished by cell markers [172, 173]. Cell markers refer to biochemical indicators that can objectively measure and evaluate cells [174, 175]. Examination of cell markers with certain specificity plays an important role in the identification, early diagnosis and prevention of diseases, and therapeutic monitoring. At present, the measure- ment and characterization of cell markers has gradually become an important hot spot in research. Cell markers are classified into extrinsic biochemical markers [ 176] and intrinsic markers [177]. The status of cells can be identified by external biochemical markers (e.g., fluorescent pigments, quantum

Published: Jan 1, 2020

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