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A Complete Guide to Gene Cloning: From Basic to Advanced DNA Cutters in Recombinant DNA Technology

A Complete Guide to Gene Cloning: From Basic to Advanced : DNA Cutters in Recombinant DNA Technology [Restriction enzymes are special types of endonucleases that have become a very valuable tool to the molecular biologist. These are a part of the defence mechanism in certain bacteria and archaea and consist of restriction modification systems. Known as cutters for recombinant DNA technology, today restriction enzymes have become an inevitable tool for successful cloning processes. This chapter elaborates and describes the various types of restriction enzymes, their mechanisms of action and applications.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Complete Guide to Gene Cloning: From Basic to Advanced DNA Cutters in Recombinant DNA Technology

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-96850-2
Pages
57 –68
DOI
10.1007/978-3-030-96851-9_4
Publisher site
See Chapter on Publisher Site

Abstract

[Restriction enzymes are special types of endonucleases that have become a very valuable tool to the molecular biologist. These are a part of the defence mechanism in certain bacteria and archaea and consist of restriction modification systems. Known as cutters for recombinant DNA technology, today restriction enzymes have become an inevitable tool for successful cloning processes. This chapter elaborates and describes the various types of restriction enzymes, their mechanisms of action and applications.]

Published: Apr 27, 2022

Keywords: Types of enzymes; Classification of enzymes; Restriction mapping; RFPL; Novel restriction enzymes

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