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A Complete Guide to Gene Cloning: From Basic to Advanced Fundamental Techniques of Recombinant DNA Transfer

A Complete Guide to Gene Cloning: From Basic to Advanced : Fundamental Techniques of Recombinant... [The delivery of a vector carrying the insert gene is one of the vital steps in DNA cloning so as to obtain multiple copies of recombinant DNA, analyze gene function, allows expression of proteins, or permits integration in host genome. The deliberate introduction of foreign DNA into cells is called transfection. For transferring of these DNA, several powerful techniques are employed. These techniques are extremely relevant because both the DNA, and the membrane that it has to cross in order to reach inside the cells are negatively charged. These gene transfer techniques usually mask the negatively charged DNA by enveloping it inside a virus or bacteria (biological method), coating or neutralizing the negative charge (chemical method) or propel the DNA through the membrane by punching holes on it (physical method). With the advances in recombinant DNA technology (RDT) various transfection methods can be applied for different cell types depending on the objective of the study. This chapter describes different methods of transfection in detail.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Complete Guide to Gene Cloning: From Basic to Advanced Fundamental Techniques of Recombinant DNA Transfer

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-96850-2
Pages
79 –95
DOI
10.1007/978-3-030-96851-9_6
Publisher site
See Chapter on Publisher Site

Abstract

[The delivery of a vector carrying the insert gene is one of the vital steps in DNA cloning so as to obtain multiple copies of recombinant DNA, analyze gene function, allows expression of proteins, or permits integration in host genome. The deliberate introduction of foreign DNA into cells is called transfection. For transferring of these DNA, several powerful techniques are employed. These techniques are extremely relevant because both the DNA, and the membrane that it has to cross in order to reach inside the cells are negatively charged. These gene transfer techniques usually mask the negatively charged DNA by enveloping it inside a virus or bacteria (biological method), coating or neutralizing the negative charge (chemical method) or propel the DNA through the membrane by punching holes on it (physical method). With the advances in recombinant DNA technology (RDT) various transfection methods can be applied for different cell types depending on the objective of the study. This chapter describes different methods of transfection in detail.]

Published: Apr 27, 2022

Keywords: Gene transfer; Transfection; Biolistic gene transfer; Liposomes; Agrobacterium -mediated gene transfer; Magnetofection; Sonoporation

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