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A Complete Guide to Gene Cloning: From Basic to Advanced Gene Isolation Methods: Beginner’s Guide

A Complete Guide to Gene Cloning: From Basic to Advanced : Gene Isolation Methods: Beginner’s Guide [As the structure of DNA is fundamentally the same in all living organisms, theoretically any segment of DNA from any organism can be introduced in any host resulting in recombinant DNA. But the trick lies in picking the right DNA fragment and inserting at the right place in a vector to successfully produce a recombinant plasmid. Molecular cloning involves several tools and techniques to introduce DNA as an insert into a vector molecule. The insert DNA can be obtained from genomic DNA by cutting it using restriction enzymes, can be amplified by PCR or copying it from an RNA template by RT-PCR. The insert DNA can also be synthesized artificially enabling to build oligos with nonnatural nucleotides. This chapter covers the methods available to obtain insert DNA.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Complete Guide to Gene Cloning: From Basic to Advanced Gene Isolation Methods: Beginner’s Guide

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-96850-2
Pages
43 –55
DOI
10.1007/978-3-030-96851-9_3
Publisher site
See Chapter on Publisher Site

Abstract

[As the structure of DNA is fundamentally the same in all living organisms, theoretically any segment of DNA from any organism can be introduced in any host resulting in recombinant DNA. But the trick lies in picking the right DNA fragment and inserting at the right place in a vector to successfully produce a recombinant plasmid. Molecular cloning involves several tools and techniques to introduce DNA as an insert into a vector molecule. The insert DNA can be obtained from genomic DNA by cutting it using restriction enzymes, can be amplified by PCR or copying it from an RNA template by RT-PCR. The insert DNA can also be synthesized artificially enabling to build oligos with nonnatural nucleotides. This chapter covers the methods available to obtain insert DNA.]

Published: Apr 27, 2022

Keywords: Genome library; cDNA library; DNA extraction; PCR; Artificial DNA synthesis

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