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A Complete Guide to Gene Cloning: From Basic to Advanced Overview of Gene Cloning Strategies

A Complete Guide to Gene Cloning: From Basic to Advanced : Overview of Gene Cloning Strategies [With the advent of DNA manipulation techniques, it is possible to generate identical copies of desired genes. These copies are introduced into a suitable vector for proliferation and can also be modified in vitro, altering the expression and function to obtain the required product. These cloning methods undergo advancements continuously and therefore various strategies have been designed to transfer sequences of interest efficiently. Recombinant DNA technology (RDT) has also enabled researchers to recognize the utility of genes in various fields of basic research, agriculture, and biomedical engineering. Here we discuss different cloning strategies that are established to successfully carry out gene transfer.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Complete Guide to Gene Cloning: From Basic to Advanced Overview of Gene Cloning Strategies

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-96850-2
Pages
69 –78
DOI
10.1007/978-3-030-96851-9_5
Publisher site
See Chapter on Publisher Site

Abstract

[With the advent of DNA manipulation techniques, it is possible to generate identical copies of desired genes. These copies are introduced into a suitable vector for proliferation and can also be modified in vitro, altering the expression and function to obtain the required product. These cloning methods undergo advancements continuously and therefore various strategies have been designed to transfer sequences of interest efficiently. Recombinant DNA technology (RDT) has also enabled researchers to recognize the utility of genes in various fields of basic research, agriculture, and biomedical engineering. Here we discuss different cloning strategies that are established to successfully carry out gene transfer.]

Published: Apr 27, 2022

Keywords: PCR cloning; Restriction and ligation-based cloning; Ligation-independent cloning; Recombinational cloning; Mating-Assisted Genetically Integrated Cloning

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