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A Complete Guide to Gene Cloning: From Basic to Advanced Recent Trends and Advances

A Complete Guide to Gene Cloning: From Basic to Advanced : Recent Trends and Advances [Targeted customization of genome has given us the ability to study gene function, make highly specific correction in genes or even introduce or insert DNA sequence. A variety of enzymes, transcription factors, and DNA-binding motifs have been engineered to achieve precision and tight regulation of modifying genome or gene expression. The key players of gene editing required components to fetch target sites and DNA cleavage enzymes that cut DNA which can be repaired or replaced leading to desired gene editing. Gene editing includes technologies like Cre-lox systems, RNA interference, CRISPR-Cas9 system, etc. This chapter will cover the details of these gene editing mechanisms.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Complete Guide to Gene Cloning: From Basic to Advanced Recent Trends and Advances

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-96850-2
Pages
119 –141
DOI
10.1007/978-3-030-96851-9_8
Publisher site
See Chapter on Publisher Site

Abstract

[Targeted customization of genome has given us the ability to study gene function, make highly specific correction in genes or even introduce or insert DNA sequence. A variety of enzymes, transcription factors, and DNA-binding motifs have been engineered to achieve precision and tight regulation of modifying genome or gene expression. The key players of gene editing required components to fetch target sites and DNA cleavage enzymes that cut DNA which can be repaired or replaced leading to desired gene editing. Gene editing includes technologies like Cre-lox systems, RNA interference, CRISPR-Cas9 system, etc. This chapter will cover the details of these gene editing mechanisms.]

Published: Apr 27, 2022

Keywords: Gene editing; Zinc finger nucleases; Transcription activator-like effectors nuclease; RNA interference; CRISPR-Cas9; Cre-Lox

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