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A Complete Guide to Gene Cloning: From Basic to Advanced Vectors: Guide to Gene Delivery Vehicles

A Complete Guide to Gene Cloning: From Basic to Advanced : Vectors: Guide to Gene Delivery Vehicles [Cloning vector is a DNA molecule acting as a vehicle to introduce foreign DNA into a desired host cell. Cloning vector enables amplification, and expression of the foreign DNA within the host cell. Over the years, multiple types of cloning vectors have been developed to cater to the need of experiments. This chapter elaborates on different types of vectors used for DNA cloning such as plasmid, cosmid, phagemid, Yeast artificial chromosomes, Bacterial artificial chromosomes, Human artificial chromosomes, and viral vectors. This chapter discusses various vectors, their features, applications advantages and disadvantages. The chapter also elaborates on vectors designed for special purposes such as expression vector and shuttle vector.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Complete Guide to Gene Cloning: From Basic to Advanced Vectors: Guide to Gene Delivery Vehicles

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-96850-2
Pages
15 –42
DOI
10.1007/978-3-030-96851-9_2
Publisher site
See Chapter on Publisher Site

Abstract

[Cloning vector is a DNA molecule acting as a vehicle to introduce foreign DNA into a desired host cell. Cloning vector enables amplification, and expression of the foreign DNA within the host cell. Over the years, multiple types of cloning vectors have been developed to cater to the need of experiments. This chapter elaborates on different types of vectors used for DNA cloning such as plasmid, cosmid, phagemid, Yeast artificial chromosomes, Bacterial artificial chromosomes, Human artificial chromosomes, and viral vectors. This chapter discusses various vectors, their features, applications advantages and disadvantages. The chapter also elaborates on vectors designed for special purposes such as expression vector and shuttle vector.]

Published: Apr 27, 2022

Keywords: Plasmid vector; Virus vectors; Artificial chromosome; Shuttle vector; Expression vectors

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