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A Guide to Methods in the Biomedical SciencesAntibody-Based Techniques

A Guide to Methods in the Biomedical Sciences: Antibody-Based Techniques Chapter 4 A. Introduction Antibodies are a group of serum glycoproteins of related structure that help protect us against invading pathogens. Antibodies are highly spe- cific for the immunogen, and generally bind with high affinity to antigenic determinants, known as epitopes, on the immunogen. Antibodies that have been produced against an immunogen can be purified from serum using, for example, affinity chromatography. These purified antibodies are generally heterogeneous in that they recognize different epitopes on the immunogen and bind with different affinities. These serum an- tibodies are referred to as polyclonal antibodies since they have been produced by many different clones of antibody secreting cells. Antibody secreting cells, also referred to as plasma cells, differentiate from B lym- phocytes in response to foreign antigens. Antibodies are crucial to the clearance of many pathogens like viruses and bacteria from our bodies. The most remarkable feature of antibodies is their ability to be produced against almost any type of macromolecule, especially proteins and car- bohydrates, whether these are naturally occurring or synthesized de novo in the laboratory. Major discoveries on the role of antibodies in adaptive immune re- sponses have been met with a number of Nobel prizes to investiga- tors http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Guide to Methods in the Biomedical SciencesAntibody-Based Techniques

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Publisher
Springer US
Copyright
© Springer Science + Business Media, Inc. 2005
ISBN
978-0-387-22844-0
Pages
69 –85
DOI
10.1007/0-387-22845-4_4
Publisher site
See Chapter on Publisher Site

Abstract

Chapter 4 A. Introduction Antibodies are a group of serum glycoproteins of related structure that help protect us against invading pathogens. Antibodies are highly spe- cific for the immunogen, and generally bind with high affinity to antigenic determinants, known as epitopes, on the immunogen. Antibodies that have been produced against an immunogen can be purified from serum using, for example, affinity chromatography. These purified antibodies are generally heterogeneous in that they recognize different epitopes on the immunogen and bind with different affinities. These serum an- tibodies are referred to as polyclonal antibodies since they have been produced by many different clones of antibody secreting cells. Antibody secreting cells, also referred to as plasma cells, differentiate from B lym- phocytes in response to foreign antigens. Antibodies are crucial to the clearance of many pathogens like viruses and bacteria from our bodies. The most remarkable feature of antibodies is their ability to be produced against almost any type of macromolecule, especially proteins and car- bohydrates, whether these are naturally occurring or synthesized de novo in the laboratory. Major discoveries on the role of antibodies in adaptive immune re- sponses have been met with a number of Nobel prizes to investiga- tors

Published: Jan 1, 2005

Keywords: Surface Plasmon Resonance; Fluorescence Activate Cell Sorting; ELISPOT Assay; Antibody Molecule; Fluorescence Activate Cell Sorting Analysis

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