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A Guide to Methods in the Biomedical SciencesDetection and Analysis of Nucleic Acids

A Guide to Methods in the Biomedical Sciences: Detection and Analysis of Nucleic Acids Chapter 2 A. Introduction The year 2003 marked the anniversary of the paper by James Watson and Francis Crick reporting the solution of the three dimensional structure of deoxyribonucleic acid (DNA) (9). This achievement earned them a Nobel Prize, which they shared in 1962 with Maurice Wilkins for his crystallographic studies on DNA structure (Table 2). It also set the stage for a new era which led to breakthroughs in our understanding of the genetic code and the development of techniques that allowed for the rapid sequencing of DNA. Eventually, the entire genome of a number of organisms would be sequenced, culminating in the complete sequenc- ing of the human genome in 1991. An understanding of DNA structure also permitted the development of methods to clone and manipulate DNA (recombinant DNA technology), as well as alter the sequence of DNA using site directed mutagenesis to gain insights into the function of individual proteins. The advent of the molecular biology revolution also allowed us to begin to understand how genes are regulated. The regu- lation of gene expression is not only the key to our understanding of how genes are turned on and off, but to how genes are regulated http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Guide to Methods in the Biomedical SciencesDetection and Analysis of Nucleic Acids

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Publisher
Springer US
Copyright
© Springer Science + Business Media, Inc. 2005
ISBN
978-0-387-22844-0
Pages
25 –38
DOI
10.1007/0-387-22845-4_2
Publisher site
See Chapter on Publisher Site

Abstract

Chapter 2 A. Introduction The year 2003 marked the anniversary of the paper by James Watson and Francis Crick reporting the solution of the three dimensional structure of deoxyribonucleic acid (DNA) (9). This achievement earned them a Nobel Prize, which they shared in 1962 with Maurice Wilkins for his crystallographic studies on DNA structure (Table 2). It also set the stage for a new era which led to breakthroughs in our understanding of the genetic code and the development of techniques that allowed for the rapid sequencing of DNA. Eventually, the entire genome of a number of organisms would be sequenced, culminating in the complete sequenc- ing of the human genome in 1991. An understanding of DNA structure also permitted the development of methods to clone and manipulate DNA (recombinant DNA technology), as well as alter the sequence of DNA using site directed mutagenesis to gain insights into the function of individual proteins. The advent of the molecular biology revolution also allowed us to begin to understand how genes are regulated. The regu- lation of gene expression is not only the key to our understanding of how genes are turned on and off, but to how genes are regulated

Published: Jan 1, 2005

Keywords: Single Nucleotide Polymorphism; Nobel Prize; Dideoxy Sequencing; Single Nucleotide Polymorphism Genotype; Protected Fragment

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