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A Guide to Methods in the Biomedical SciencesMicroscopy: Imaging of Biological Specimens

A Guide to Methods in the Biomedical Sciences: Microscopy: Imaging of Biological Specimens Chapter 5 MICROSCOPY: IMAGING OF BIOLOGICAL SPECIMENS A. Introduction Imaging technologies seek to peer inside tissues, cells, or even an en- tire organism, including man, to reveal underlying structures. The most common methods of imaging use the microscope. The use of micro- scopes has become an essential part of almost every field of biomedical sciences, and is particularly important in the field of Cell Biology, which seeks to understand how cells are organized and function. Anton van Leeuwenhoek of Holland is generally considered to be the father of the microscope. The first “light microscope” was invented by him in the mid century. Convex lenses, the essential compo- nent required for magnification, had been developed about 100 years earlier. The single lens design limited the amount of magnification pos- sible. The development of the compound microscope late in the century helped overcome this limitation. A compound microscope is a microscope consisting of 2 lenses, one the “objective” and the second the “eyepiece”. It was with these early microscopes that the Englishman Robert Hooke looked at the unseen world and gave the name “cell” to de- scribe the smallest observable structures that made up living organisms. Later, Louis Pasteur was able http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Guide to Methods in the Biomedical SciencesMicroscopy: Imaging of Biological Specimens

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Publisher
Springer US
Copyright
© Springer Science + Business Media, Inc. 2005
ISBN
978-0-387-22844-0
Pages
87 –99
DOI
10.1007/0-387-22845-4_5
Publisher site
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Abstract

Chapter 5 MICROSCOPY: IMAGING OF BIOLOGICAL SPECIMENS A. Introduction Imaging technologies seek to peer inside tissues, cells, or even an en- tire organism, including man, to reveal underlying structures. The most common methods of imaging use the microscope. The use of micro- scopes has become an essential part of almost every field of biomedical sciences, and is particularly important in the field of Cell Biology, which seeks to understand how cells are organized and function. Anton van Leeuwenhoek of Holland is generally considered to be the father of the microscope. The first “light microscope” was invented by him in the mid century. Convex lenses, the essential compo- nent required for magnification, had been developed about 100 years earlier. The single lens design limited the amount of magnification pos- sible. The development of the compound microscope late in the century helped overcome this limitation. A compound microscope is a microscope consisting of 2 lenses, one the “objective” and the second the “eyepiece”. It was with these early microscopes that the Englishman Robert Hooke looked at the unseen world and gave the name “cell” to de- scribe the smallest observable structures that made up living organisms. Later, Louis Pasteur was able

Published: Jan 1, 2005

Keywords: Fluorescence Resonance Energy Transfer; Nobel Prize; Total Internal Reflection; Differential Interference Contrast; Fluorescence Recovery After Photobleaching

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