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A Guide to Methods in the Biomedical SciencesRecombinant DNA Techniques: Cloning and Manipulation of DNA

A Guide to Methods in the Biomedical Sciences: Recombinant DNA Techniques: Cloning and... Chapter 3 RECOMBINANT DNA TECHNIQUES: CLONING AND MANIPULATION OF DNA A. Introduction Recombinant DNA refers to DNA that has been experimentally ma- nipulated in a laboratory by adding or deleting genes. Recombinant DNA is usually incorporated into vectors that can be introduced into bacte- ria, so that the bacteria now contains the gene(s) of interest, genes that they would not normally express. The advent of recombinant DNA technology is one of the most significant developments of century biomedical science. The ability to clone genes led to the establishment of the Biotechnology Industry, which uses cloned genes for enzyme and hormone replacement therapies. For example, before the advent of the biotechnology industry, insulin was purified from natural sources, limiting its availability. Now, it can be produced in essentially limitless quantities and at significantly reduced cost. Recombinant DNA techniques are used to establish new therapies for treating diseases, such as immun- odeficiency diseases and metabolic disorders, and are also being used for vaccine development. Recombinant DNA techniques have also sig- nificantly enhanced our ability to analyze protein structure and function, determine how genes turn on and off, and understand tissue specific gene expression. The ability to clone DNA allows us to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Guide to Methods in the Biomedical SciencesRecombinant DNA Techniques: Cloning and Manipulation of DNA

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Publisher
Springer US
Copyright
© Springer Science + Business Media, Inc. 2005
ISBN
978-0-387-22844-0
Pages
39 –68
DOI
10.1007/0-387-22845-4_3
Publisher site
See Chapter on Publisher Site

Abstract

Chapter 3 RECOMBINANT DNA TECHNIQUES: CLONING AND MANIPULATION OF DNA A. Introduction Recombinant DNA refers to DNA that has been experimentally ma- nipulated in a laboratory by adding or deleting genes. Recombinant DNA is usually incorporated into vectors that can be introduced into bacte- ria, so that the bacteria now contains the gene(s) of interest, genes that they would not normally express. The advent of recombinant DNA technology is one of the most significant developments of century biomedical science. The ability to clone genes led to the establishment of the Biotechnology Industry, which uses cloned genes for enzyme and hormone replacement therapies. For example, before the advent of the biotechnology industry, insulin was purified from natural sources, limiting its availability. Now, it can be produced in essentially limitless quantities and at significantly reduced cost. Recombinant DNA techniques are used to establish new therapies for treating diseases, such as immun- odeficiency diseases and metabolic disorders, and are also being used for vaccine development. Recombinant DNA techniques have also sig- nificantly enhanced our ability to analyze protein structure and function, determine how genes turn on and off, and understand tissue specific gene expression. The ability to clone DNA allows us to

Published: Jan 1, 2005

Keywords: Polymerase Chain Reaction; Bacterial Artificial Chromosome; Electrophoretic Mobility Shift Assay; Biomedical Science; Crime Scene

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