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A Handbook of Transcription FactorsIdentification of Transcription Factor–DNA Interactions In Vivo

A Handbook of Transcription Factors: Identification of Transcription Factor–DNA Interactions In Vivo [Recent technological developments have revolutionized our understanding of transcriptional regulation by providing an unprecedented ability to interrogate in vivo transcription factor binding. The combination of high-throughput sequencing with chromatin precipitation of transcription factors and specifically labeled histones has allowed direct protein-DNA contacts to be visualized across genomes as large and complex as mammals at base-pair resolution. This chapter reviews the developments that led to these insights, with particular focus on examples of early protein-DNA localization experiments using genomic microarrays in mammals and yeast. Four state-of-the-art research directions are highlighted as examples of previously unimaginable frontiers now under active investigation.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Handbook of Transcription FactorsIdentification of Transcription Factor–DNA Interactions In Vivo

Part of the Subcellular Biochemistry Book Series (volume 52)
Editors: Hughes, Timothy R.

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References (67)

Publisher
Springer Netherlands
Copyright
© Springer Science+Business Media B.V. 2011
ISBN
978-90-481-9068-3
Pages
175 –191
DOI
10.1007/978-90-481-9069-0_8
Publisher site
See Chapter on Publisher Site

Abstract

[Recent technological developments have revolutionized our understanding of transcriptional regulation by providing an unprecedented ability to interrogate in vivo transcription factor binding. The combination of high-throughput sequencing with chromatin precipitation of transcription factors and specifically labeled histones has allowed direct protein-DNA contacts to be visualized across genomes as large and complex as mammals at base-pair resolution. This chapter reviews the developments that led to these insights, with particular focus on examples of early protein-DNA localization experiments using genomic microarrays in mammals and yeast. Four state-of-the-art research directions are highlighted as examples of previously unimaginable frontiers now under active investigation.]

Published: Apr 14, 2011

Keywords: Protein-DNA contact; Chromatin immunoprecipitation; Transcription; Transcriptional regulation; Gene expression

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