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Characterization of the two nonallelic genes encoding mouse preproinsulin

Characterization of the two nonallelic genes encoding mouse preproinsulin We have cloned and sequenced the two mouse preproinsulin genes. The deduced amino acid sequences of the mature mouse insulins are identical to the published protein sequences. However, the nucleotide sequence indicates that the mouse I C-peptide has a deletion of two amino acids compared with the mouse II C-peptide. We used an S1 nuclease assay to confirm the presence of the deletion and to measure the ratio of transcripts from gene I to transcripts from gene II. The mouse preproinsulin I gene, like the rat gene I, is missing the second intervening sequence that normally interrupts the C-peptide region in other insulin genes. Comparison of the 5′ flanking sequences of the mouse and rat genes II indicates that they are homologous for at least 1000 base pairs. The preproinsulin I genes also share homology in their 5′ flanking DNAs; however, their homology to the preproinsulin II genes extends for only about 500 base pairs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Molecular Evolution Springer Journals

Characterization of the two nonallelic genes encoding mouse preproinsulin

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References (53)

Publisher
Springer Journals
Copyright
Copyright © 1986 by Springer-Verlag New York Inc.
Subject
Life Sciences; Evolutionary Biology; Microbiology; Plant Sciences; Plant Genetics and Genomics; Animal Genetics and Genomics; Cell Biology
ISSN
0022-2844
eISSN
1432-1432
DOI
10.1007/BF02100639
Publisher site
See Article on Publisher Site

Abstract

We have cloned and sequenced the two mouse preproinsulin genes. The deduced amino acid sequences of the mature mouse insulins are identical to the published protein sequences. However, the nucleotide sequence indicates that the mouse I C-peptide has a deletion of two amino acids compared with the mouse II C-peptide. We used an S1 nuclease assay to confirm the presence of the deletion and to measure the ratio of transcripts from gene I to transcripts from gene II. The mouse preproinsulin I gene, like the rat gene I, is missing the second intervening sequence that normally interrupts the C-peptide region in other insulin genes. Comparison of the 5′ flanking sequences of the mouse and rat genes II indicates that they are homologous for at least 1000 base pairs. The preproinsulin I genes also share homology in their 5′ flanking DNAs; however, their homology to the preproinsulin II genes extends for only about 500 base pairs.

Journal

Journal of Molecular EvolutionSpringer Journals

Published: Aug 2, 2005

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