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Development of the paper-based colorimetric sensor for simple and fast determination of quercetin in guava leaf extract

Development of the paper-based colorimetric sensor for simple and fast determination of quercetin... This present study aimed to develop a paper-based colorimetric sensor in the form of paper-microzone plates (PµZP), for simple and fast quercetin determination in guava leaf extract samples. Here, N-bromosuccinimide (NBS) solution was immobilized on the microzone as a sensing probe, where quercetin solution can be dropped on it to form red-purplish color adducts which can be seen by the naked eye or captured using a flatbed scanner. The color intensity of the microzone can be quantified against a blank solution and used as analytical data in scanometric assay. The sensor showed a response time of 8 min, a linear interval of 1–10 mM with a detection limit at 1.274 mM toward quercetin, and exhibited good reproducibility (RSD < 1%) and accuracy (98–99% recovery). The quercetin level of guava leaf extract determined by the PµZP-scanometric method was found comparable with that of the TLC-densitometric method, suggesting its use as an alternative method for quercetin analysis in the guava leaf extract.Graphical abstract[graphic not available: see fulltext] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Sciences Springer Journals

Development of the paper-based colorimetric sensor for simple and fast determination of quercetin in guava leaf extract

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References (33)

Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
0910-6340
eISSN
1348-2246
DOI
10.1007/s44211-023-00380-y
Publisher site
See Article on Publisher Site

Abstract

This present study aimed to develop a paper-based colorimetric sensor in the form of paper-microzone plates (PµZP), for simple and fast quercetin determination in guava leaf extract samples. Here, N-bromosuccinimide (NBS) solution was immobilized on the microzone as a sensing probe, where quercetin solution can be dropped on it to form red-purplish color adducts which can be seen by the naked eye or captured using a flatbed scanner. The color intensity of the microzone can be quantified against a blank solution and used as analytical data in scanometric assay. The sensor showed a response time of 8 min, a linear interval of 1–10 mM with a detection limit at 1.274 mM toward quercetin, and exhibited good reproducibility (RSD < 1%) and accuracy (98–99% recovery). The quercetin level of guava leaf extract determined by the PµZP-scanometric method was found comparable with that of the TLC-densitometric method, suggesting its use as an alternative method for quercetin analysis in the guava leaf extract.Graphical abstract[graphic not available: see fulltext]

Journal

Analytical Sciences – Springer Journals

Published: Oct 1, 2023

Keywords: NBS; Quercetin; Colorimetric sensor; Guava leaf extract

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