Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars

Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars Genotypic restrictions on plant regeneration from cultured cells have hindered the genetic transformation of most barley cultivars. Optimizing culturing protocols for specific cultivars of commercial interest may facilitate their genetic transformation. Plant regeneration from embryogenic callus of `Harrington', `Morex', and `Hector' as affected by certain protocol modifications was examined in replicated experiments. Regeneration was improved for all cultivars by separately autoclaving certain components of the culture media and by reducing the amount of embryogenic callus cultured per petri dish. Regeneration improvements in response to various concentrations of copper and 2,4-dichlorophenoxyacetic acid were more genotype specific. This study suggests that the development and use of genotype-specific protocols can enhance plant regeneration. Enhancements in plant regeneration are expected to facilitate the transformation of commercial barley germplasm. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars

Plant Cell Reports , Volume 17 (12) – Sep 1, 1998

Loading next page...
 
/lp/springer-journals/enhancement-of-plant-regeneration-from-embryogenic-callus-of-qRgEXVuglc

References (6)

Publisher
Springer Journals
Copyright
Copyright © 1998 by Springer-Verlag Berlin Heidelberg
Subject
Legacy
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s002990050514
Publisher site
See Article on Publisher Site

Abstract

Genotypic restrictions on plant regeneration from cultured cells have hindered the genetic transformation of most barley cultivars. Optimizing culturing protocols for specific cultivars of commercial interest may facilitate their genetic transformation. Plant regeneration from embryogenic callus of `Harrington', `Morex', and `Hector' as affected by certain protocol modifications was examined in replicated experiments. Regeneration was improved for all cultivars by separately autoclaving certain components of the culture media and by reducing the amount of embryogenic callus cultured per petri dish. Regeneration improvements in response to various concentrations of copper and 2,4-dichlorophenoxyacetic acid were more genotype specific. This study suggests that the development and use of genotype-specific protocols can enhance plant regeneration. Enhancements in plant regeneration are expected to facilitate the transformation of commercial barley germplasm.

Journal

Plant Cell ReportsSpringer Journals

Published: Sep 1, 1998

There are no references for this article.