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Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L.)

Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena... A reliable and efficient protocol for the regeneration of fertile plants derived from leaf base segments of young in-vitro-grown oat seedlings has been developed successfully. Callus induction and shoot regeneration were achieved when the basal region of young seedlings was cultured on auxin-containing medium. Callus induction efficiencies as well as regeneration frequencies were correlated with the developmental stage and the genotype of the explants. In five different genotypes of oat, an average of 25 plants per explant could be produced and for the most responsive genotype more than 50 regenerants per explant could be regenerated reproducibly. This high regeneration potential makes oat leaf bases a very attractive target for transformation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L.)

Plant Cell Reports , Volume 17 (6) – Apr 1, 1998

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References (14)

Publisher
Springer Journals
Copyright
Copyright © 1998 by Springer-Verlag Berlin Heidelberg
Subject
Legacy
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s002990050422
Publisher site
See Article on Publisher Site

Abstract

A reliable and efficient protocol for the regeneration of fertile plants derived from leaf base segments of young in-vitro-grown oat seedlings has been developed successfully. Callus induction and shoot regeneration were achieved when the basal region of young seedlings was cultured on auxin-containing medium. Callus induction efficiencies as well as regeneration frequencies were correlated with the developmental stage and the genotype of the explants. In five different genotypes of oat, an average of 25 plants per explant could be produced and for the most responsive genotype more than 50 regenerants per explant could be regenerated reproducibly. This high regeneration potential makes oat leaf bases a very attractive target for transformation.

Journal

Plant Cell ReportsSpringer Journals

Published: Apr 1, 1998

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