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GlycoinformaticsExploring the Specificities of Glycan-Binding Proteins Using Glycan Array Data and the GlycoSearch Software

Glycoinformatics: Exploring the Specificities of Glycan-Binding Proteins Using Glycan Array Data... [The glycan array is a powerful tool for investigating the specificities of glycan-binding proteins. By incubating a glycan-binding protein on a glycan array, the relative binding to hundreds of different oligosaccharides can be quantified in parallel. Based on these data, much information can be obtained about the preference of a glycan-binding protein for specific subcomponents of oligosaccharides or motifs. In many cases, the analysis and interpretation of glycan array data can be time consuming and imprecise if done manually. Recently we developed software, called GlycoSearch, to facilitate the analysis and interpretation of glycan array data based on the previously developed methods called Motif Segregation and Outlier Motif Analysis. Here we describe the principles behind the software, the use of the software, and an example application. The automated, objective, and precise analysis of glycan array data should enhance the value of the data for a broad range of research applications.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

GlycoinformaticsExploring the Specificities of Glycan-Binding Proteins Using Glycan Array Data and the GlycoSearch Software

Part of the Methods in Molecular Biology Book Series (volume 1273)
Editors: Lütteke, Thomas; Frank, Martin
Glycoinformatics — Feb 13, 2015

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References (11)

Publisher
Springer New York
Copyright
© Springer Science+Business Media New York 2015
ISBN
978-1-4939-2342-7
Pages
203 –214
DOI
10.1007/978-1-4939-2343-4_15
Publisher site
See Chapter on Publisher Site

Abstract

[The glycan array is a powerful tool for investigating the specificities of glycan-binding proteins. By incubating a glycan-binding protein on a glycan array, the relative binding to hundreds of different oligosaccharides can be quantified in parallel. Based on these data, much information can be obtained about the preference of a glycan-binding protein for specific subcomponents of oligosaccharides or motifs. In many cases, the analysis and interpretation of glycan array data can be time consuming and imprecise if done manually. Recently we developed software, called GlycoSearch, to facilitate the analysis and interpretation of glycan array data based on the previously developed methods called Motif Segregation and Outlier Motif Analysis. Here we describe the principles behind the software, the use of the software, and an example application. The automated, objective, and precise analysis of glycan array data should enhance the value of the data for a broad range of research applications.]

Published: Feb 13, 2015

Keywords: Glycan arrays; Motif analysis; Lectins; GlycoSearch

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