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GlycosaminoglycansKeratan Sulfate: Biosynthesis, Structures, and Biological Functions

Glycosaminoglycans: Keratan Sulfate: Biosynthesis, Structures, and Biological Functions [Keratan sulfate is a glycosaminoglycan that has been investigated in the cornea and skeletal tissues for decades. Endoglycosidases and monoclonal antibodies specific for keratan sulfate have been developed. These materials have facilitated the analysis of keratan sulfate biosynthesis and structures. Likewise, they have expedited study of the biological roles of keratan sulfate in vitro and in vivo. It has been shown that keratan sulfate is also expressed in the central nervous system and functions as a regulator of neuronal regeneration/sprouting. Here, we describe methods to determine the enzymatic activity of GlcNAc6ST, which is involved in keratan sulfate biosynthesis, and to extract and prepare ocular keratan sulfate for a disaccharide composition analysis. Immunohistochemistry for an anti-keratan sulfate epitope in the brain is also described.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

GlycosaminoglycansKeratan Sulfate: Biosynthesis, Structures, and Biological Functions

Part of the Methods in Molecular Biology Book Series (volume 1229)
Editors: Balagurunathan, Kuberan; Nakato, Hiroshi; Desai, Umesh R.
Glycosaminoglycans — Sep 24, 2014

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References (41)

Publisher
Springer New York
Copyright
© Springer Science+Business Media New York 2015
ISBN
978-1-4939-1713-6
Pages
389 –400
DOI
10.1007/978-1-4939-1714-3_30
Publisher site
See Chapter on Publisher Site

Abstract

[Keratan sulfate is a glycosaminoglycan that has been investigated in the cornea and skeletal tissues for decades. Endoglycosidases and monoclonal antibodies specific for keratan sulfate have been developed. These materials have facilitated the analysis of keratan sulfate biosynthesis and structures. Likewise, they have expedited study of the biological roles of keratan sulfate in vitro and in vivo. It has been shown that keratan sulfate is also expressed in the central nervous system and functions as a regulator of neuronal regeneration/sprouting. Here, we describe methods to determine the enzymatic activity of GlcNAc6ST, which is involved in keratan sulfate biosynthesis, and to extract and prepare ocular keratan sulfate for a disaccharide composition analysis. Immunohistochemistry for an anti-keratan sulfate epitope in the brain is also described.]

Published: Sep 24, 2014

Keywords: Keratan sulfate; Sulfotransferase; N -acetylglucosamine; Galactose; Immunohistochemistry; Disaccharide analysis; Enzymatic specificity

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