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Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.)

Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.) We report a less genotype-dependent in vitro regeneration system capable of producing multiple shoot clumps and whole plants in four different wheat genotypes. Shool apical meristems from 7-d-old-seedlings produced axillary and adventitious shoots and somatic embryos on media containing N6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). All four genotypes responded positively to shoot multiplication depending upon media composition. Scanning electron microscopies of cultures showed a proliferating budding state that gave rise to adventitious shoots and somatic embryos on further multiplication. The percentage of relative shoot apical meristem multiplication was 80–90%, and the average number of shoot meristems per multiplied shoot was 40–50 in all genotypes. Among different concentrations of phytohormones, 2 and 4 mgl−1 BA (8.8 and 17.7 μM) in combination with 0.5 mg l−1 2,4-D (2.26 μM) gave the best results. Actively multiplying shoot clumps were recovered with high frequency among 3-mo.-old cultures. These shoot clumps regenerated normally and produced fertile plants containing viable seeds. This in vitro system might prove useful for the production of transgenic plants of wheat in a relatively genotype-independent manner. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png In Vitro Cellular & Developmental Biology - Plant Springer Journals

Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.)

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References (29)

Publisher
Springer Journals
Copyright
Copyright © 2002 by Society for In Vitro Biology
Subject
Life Sciences; Cell Biology; Developmental Biology; Plant Genetics & Genomics; Plant Biochemistry; Plant Physiology
ISSN
1054-5476
eISSN
1475-2689
DOI
10.1079/IVP2001267
Publisher site
See Article on Publisher Site

Abstract

We report a less genotype-dependent in vitro regeneration system capable of producing multiple shoot clumps and whole plants in four different wheat genotypes. Shool apical meristems from 7-d-old-seedlings produced axillary and adventitious shoots and somatic embryos on media containing N6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). All four genotypes responded positively to shoot multiplication depending upon media composition. Scanning electron microscopies of cultures showed a proliferating budding state that gave rise to adventitious shoots and somatic embryos on further multiplication. The percentage of relative shoot apical meristem multiplication was 80–90%, and the average number of shoot meristems per multiplied shoot was 40–50 in all genotypes. Among different concentrations of phytohormones, 2 and 4 mgl−1 BA (8.8 and 17.7 μM) in combination with 0.5 mg l−1 2,4-D (2.26 μM) gave the best results. Actively multiplying shoot clumps were recovered with high frequency among 3-mo.-old cultures. These shoot clumps regenerated normally and produced fertile plants containing viable seeds. This in vitro system might prove useful for the production of transgenic plants of wheat in a relatively genotype-independent manner.

Journal

In Vitro Cellular & Developmental Biology - PlantSpringer Journals

Published: Feb 20, 2007

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