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- Publisher
- Springer International Publishing
- Copyright
- © Springer Nature Switzerland AG 2021
- ISBN
- 978-3-030-77790-6
- Pages
- 89 –126
- DOI
- 10.1007/978-3-030-77791-3_5
- Publisher site
- See Chapter on Publisher Site
Abstract
[The description of a complex carbohydrate structure includes, in the first instance, monosaccharide composition, anomeric configurations, and the type of glycosidic linkages. To obtain these data, several techniques are available. This chapter describes chemical solvolytic cleavage methods to generate monosaccharides and/or oligosaccharide fragments and discusses, in detail, the chromatographic separation methods to isolate them for further study. Several detection techniques are discussed. Electrophoretic separation of carbohydrates is briefly discussed. A typical flow chart for the structural analysis of carbohydrates from biological samples is presented. Furthermore, protocols for fluorescent or chromophore labeling of glycans are provided.]
Published: Oct 24, 2021
Keywords: Glycomics; HPLC; HPAEC-PAD; MALDI-TOF-MS; ESI-MS; GC-MS; NMR; TLC; SEC; HILIC; PGC; CZE; Glycosidic bond; Hydrolysis; Methanolysis; Formolysis; Acetolysis; Periodate oxidation; IgG; Affinity chromatography; Lectins; Reductive amination; Michael addition; PMP labeling; Permethylation