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[For studies of glycan structure/function relationships of glycoconjugates, a detailed characterization of the carbohydrate moiety is a prerequisite. To this end, the glycans are usually released and isolated before analysis. This chapter provides protocols for the chemical and enzymatic release of different types of glycans from glycoconjugates and discusses the pro and cons of the different methods. A typical strategy for the analysis of glycoproteins is described. The preparation of glycopeptides and their isolation might form part of this process. Furthermore, a typical scheme for the analysis of proteoglycans and their glycosaminoglycans is provided. Also, attention is paid to the analysis of glycolipids and polysaccharides.]
Published: Oct 24, 2021
Keywords: Glycoproteins; Glycopeptides; Proteoglycans; Glycosaminoglycans (GAG); Glycolipids; Glycosylphosphatidylinositol anchors; Glycan profiling; Hydrazinolysis; PNGase F digestion; Denaturing; Proteolysis; Reductive amination; Reduction/alkylation; β-elimination; Pronase; Trypsin; Endoglycoceramidase; Lipase; Hydrogen fluoride; Nitrous acid; Fatty acids
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