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Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue engineering

Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue... It has been hypothesized that leaflet substrates with a trilayer structure and anisotropic mechanical properties could be useful for the production of functional and long-lasting tissue-engineered leaflets. To investigate the influence of the anisotropic structural and mechanical characteristics of a substrate on cells, in this study, we electrospun trilayer anisotropic fibrous substrates and randomly oriented isotropic fibrous substrates (used as controls) from polycaprolactone polymers. Consequently, the random substrates had higher radial and lower circumferential tensile properties than the trilayer substrates; however, they had similar flexural properties. Porcine valvular interstitial cells cultured on both substrates produced random and trilayer cell-cultured constructs, respectively. The trilayer cell-cultured constructs had more anisotropic mechanical properties, 17% higher cellular proliferation, 14% more extracellular matrix (i.e., collagen and glycosaminoglycan) production, and superior gene and protein expression, suggesting that more cells were in a growth state in the trilayer constructs than in the random constructs. Furthermore, the random and radial layers of the trilayer constructs had more vimentin, collagen, transforming growth factor-beta 1 (TGF-ß1), transforming growth factor-beta 3 (TGF-ß3) gene expression than in the circumferential layer of the constructs. This study verifies that the differences in structural, tensile, and anisotropic properties of the trilayer and random substrates influence the characteristics of the cells and ECM in the constructs.Graphic abstract[graphic not available: see fulltext] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Bio-Design and Manufacturing Springer Journals

Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue engineering

Bio-Design and Manufacturing , Volume 6 (4) – Jul 1, 2023

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References (75)

Publisher
Springer Journals
Copyright
Copyright © Zhejiang University Press 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
2096-5524
eISSN
2522-8552
DOI
10.1007/s42242-023-00237-3
Publisher site
See Article on Publisher Site

Abstract

It has been hypothesized that leaflet substrates with a trilayer structure and anisotropic mechanical properties could be useful for the production of functional and long-lasting tissue-engineered leaflets. To investigate the influence of the anisotropic structural and mechanical characteristics of a substrate on cells, in this study, we electrospun trilayer anisotropic fibrous substrates and randomly oriented isotropic fibrous substrates (used as controls) from polycaprolactone polymers. Consequently, the random substrates had higher radial and lower circumferential tensile properties than the trilayer substrates; however, they had similar flexural properties. Porcine valvular interstitial cells cultured on both substrates produced random and trilayer cell-cultured constructs, respectively. The trilayer cell-cultured constructs had more anisotropic mechanical properties, 17% higher cellular proliferation, 14% more extracellular matrix (i.e., collagen and glycosaminoglycan) production, and superior gene and protein expression, suggesting that more cells were in a growth state in the trilayer constructs than in the random constructs. Furthermore, the random and radial layers of the trilayer constructs had more vimentin, collagen, transforming growth factor-beta 1 (TGF-ß1), transforming growth factor-beta 3 (TGF-ß3) gene expression than in the circumferential layer of the constructs. This study verifies that the differences in structural, tensile, and anisotropic properties of the trilayer and random substrates influence the characteristics of the cells and ECM in the constructs.Graphic abstract[graphic not available: see fulltext]

Journal

Bio-Design and ManufacturingSpringer Journals

Published: Jul 1, 2023

Keywords: Heart valve leaflet; Trilayer; Tissue engineering; Fiber; Electrospinning

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