Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Demonstration of Insulin production and storage in insulinomas by in situ hybridisation and immunocytochemistry

Demonstration of Insulin production and storage in insulinomas by in situ hybridisation and... Twelve cases of insulinoma were studied to assess the amount of hormone synthesis and hormone storage by the tumour and to see what effect a hormone‐producing tumour has on the adjacent normal islets. This was investigated by performing in situ hybridization, which detects hormone messenger RNA, thus giving an indication of the degree of hormone synthesis by the tumour, and immunocytochemistry, which detects the hormone itself, thus giving an indication of the amount of hormone stored in the tumour cells. It was found that in most cases there was less hormone stored within the tumour cells than in adjacent islet cells. In a minority of cases, this decrease in stored hormone was associated with reduced hormone synthesis, but the majority of cases showed either equivalent or increased levels of hormone mRNA in the tumour cells compared with adjacent islets. In addition, it was noted that, unlike some other endocrine organs, the presence of a hormone‐producing tumour within the pancreas did not appear to inhibit hormone synthesis in the adjacent normal tissue. © 1997 John Wiley & Sons, Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Pathology Wiley

Demonstration of Insulin production and storage in insulinomas by in situ hybridisation and immunocytochemistry

Loading next page...
 
/lp/wiley/demonstration-of-insulin-production-and-storage-in-insulinomas-by-in-GFRzZtbHA1

References (12)

Publisher
Wiley
Copyright
Copyright © 1997 Wiley Subscription Services
ISSN
0022-3417
eISSN
1096-9896
DOI
10.1002/(SICI)1096-9896(199702)181:2<218::AID-PATH732>3.0.CO;2-K
pmid
9120729
Publisher site
See Article on Publisher Site

Abstract

Twelve cases of insulinoma were studied to assess the amount of hormone synthesis and hormone storage by the tumour and to see what effect a hormone‐producing tumour has on the adjacent normal islets. This was investigated by performing in situ hybridization, which detects hormone messenger RNA, thus giving an indication of the degree of hormone synthesis by the tumour, and immunocytochemistry, which detects the hormone itself, thus giving an indication of the amount of hormone stored in the tumour cells. It was found that in most cases there was less hormone stored within the tumour cells than in adjacent islet cells. In a minority of cases, this decrease in stored hormone was associated with reduced hormone synthesis, but the majority of cases showed either equivalent or increased levels of hormone mRNA in the tumour cells compared with adjacent islets. In addition, it was noted that, unlike some other endocrine organs, the presence of a hormone‐producing tumour within the pancreas did not appear to inhibit hormone synthesis in the adjacent normal tissue. © 1997 John Wiley & Sons, Ltd.

Journal

The Journal of PathologyWiley

Published: Jan 1, 1997

Keywords: ; ; ; ; ;

There are no references for this article.