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Glycoproteomics on the rise: Established methods, advanced techniques, sophisticated biological applications

Glycoproteomics on the rise: Established methods, advanced techniques, sophisticated biological... Glycosylation is the most complex form of protein PTMs. Affected proteins may carry dozens of glycosylation sites with tens to hundreds of glycan residues attached to every site. Glycosylated proteins have many important functions in biology, from cellular to organismal levels, being involved in cell–cell signaling, cell adhesion, immune response, host–pathogen interactions, and development and growth. Glycosylation, however, expands the biological functional diversity of proteins at the expense of a tremendous increase in structural heterogeneity. Aberrant glycosylation of cell surface proteins, as well as their detectable fingerprint in plasma samples, has been associated with cancer, inflammatory and degenerative diseases, and congenital disorders of glycosylation. Therefore, there are on‐going efforts directed toward developing new technologies and approaches for glycan sequencing and high‐throughput analysis of glycosylated proteins in complex samples with simultaneous characterization of both the protein and glycan moieties. This work is aimed primarily at pinpointing the challenges associated with the large‐scale analysis of glycoproteins and the latest developments in glycoproteomic research, with focus on recent advancements (2011–2012) in microcolumn separations and MS detection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Electrophoresis Wiley

Glycoproteomics on the rise: Established methods, advanced techniques, sophisticated biological applications

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References (103)

Publisher
Wiley
Copyright
"© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim"
ISSN
0173-0835
eISSN
1522-2683
DOI
10.1002/elps.201200445
pmid
23161435
Publisher site
See Article on Publisher Site

Abstract

Glycosylation is the most complex form of protein PTMs. Affected proteins may carry dozens of glycosylation sites with tens to hundreds of glycan residues attached to every site. Glycosylated proteins have many important functions in biology, from cellular to organismal levels, being involved in cell–cell signaling, cell adhesion, immune response, host–pathogen interactions, and development and growth. Glycosylation, however, expands the biological functional diversity of proteins at the expense of a tremendous increase in structural heterogeneity. Aberrant glycosylation of cell surface proteins, as well as their detectable fingerprint in plasma samples, has been associated with cancer, inflammatory and degenerative diseases, and congenital disorders of glycosylation. Therefore, there are on‐going efforts directed toward developing new technologies and approaches for glycan sequencing and high‐throughput analysis of glycosylated proteins in complex samples with simultaneous characterization of both the protein and glycan moieties. This work is aimed primarily at pinpointing the challenges associated with the large‐scale analysis of glycoproteins and the latest developments in glycoproteomic research, with focus on recent advancements (2011–2012) in microcolumn separations and MS detection.

Journal

ElectrophoresisWiley

Published: Jan 1, 2013

Keywords: ; ;

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