Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Matrix‐assisted laser desorption/ionization mass spectrometry compatible β‐elimination of O‐linked oligosaccharides

Matrix‐assisted laser desorption/ionization mass spectrometry compatible β‐elimination of... A new β‐elimination procedure has been introduced to cleave O‐linked oligosaccharides from low‐ to sub‐microgram amounts of glycoproteins prior to analysis by mass spectrometry. Borane‐ammonia complex in aqueous ammonia is used as a cleaving solution alternative to the sodium borohydride/sodium hydroxide medium conventionally used in β‐elimination. The procedure results in minimum sample purification, leading to minimal sample loss and consequently an overall enhancement in sensitivity. It was applied successfully in the analysis of bovine fetuin and submaxillary mucin, as well as to a complex bile‐salt‐stimulated lipase glycoprotein isolated from human milk. Copyright © 2002 John Wiley & Sons, Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rapid Communications in Mass Spectrometry Wiley

Matrix‐assisted laser desorption/ionization mass spectrometry compatible β‐elimination of O‐linked oligosaccharides

Loading next page...
 
/lp/wiley/matrix-assisted-laser-desorption-ionization-mass-spectrometry-y1o57qkPJE

References (32)

Publisher
Wiley
Copyright
Copyright © 2002 Wiley Subscription Services
ISSN
0951-4198
eISSN
1097-0231
DOI
10.1002/rcm.701
pmid
12112272
Publisher site
See Article on Publisher Site

Abstract

A new β‐elimination procedure has been introduced to cleave O‐linked oligosaccharides from low‐ to sub‐microgram amounts of glycoproteins prior to analysis by mass spectrometry. Borane‐ammonia complex in aqueous ammonia is used as a cleaving solution alternative to the sodium borohydride/sodium hydroxide medium conventionally used in β‐elimination. The procedure results in minimum sample purification, leading to minimal sample loss and consequently an overall enhancement in sensitivity. It was applied successfully in the analysis of bovine fetuin and submaxillary mucin, as well as to a complex bile‐salt‐stimulated lipase glycoprotein isolated from human milk. Copyright © 2002 John Wiley & Sons, Ltd.

Journal

Rapid Communications in Mass SpectrometryWiley

Published: Jan 30, 2002

There are no references for this article.