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Multidimensional separations in top–down proteomics

Multidimensional separations in top–down proteomics Top–down proteomics (TDP) identifies, quantifies, and characterises proteins at the intact proteoform level in complex biological samples to understand proteoform function and cellular mechanisms. However, analysing complex biological samples using TDP is still challenging due to high sample complexity and wide dynamic range. High‐resolution separation methods are often applied prior to mass spectrometry (MS) analysis to decrease sample complexity and increase proteomics throughput. These separation methods, however, may not be efficient enough to characterise low abundance intact proteoforms in complex samples. As such, multidimensional separation techniques (combination of two or more separation methods with high orthogonality) have been developed and applied that demonstrate improved separation resolution and more comprehensive identification in TDP. A suite of multidimensional separation methods that couple various types of liquid chromatography (LC), capillary electrophoresis (CE), and/or gel electrophoresis‐based separation approaches have been developed and applied in TDP to analyse complex biological samples. Here, we reviewed multidimensional separation strategies employed for TDP, summarised current applications, and discussed the gaps that may be addressed in the future. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Science Advances Wiley

Multidimensional separations in top–down proteomics

23 pages

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References (135)

Publisher
Wiley
Copyright
© 2023 Wiley‐VCH GmbH.
eISSN
2628-5452
DOI
10.1002/ansa.202300016
Publisher site
See Article on Publisher Site

Abstract

Top–down proteomics (TDP) identifies, quantifies, and characterises proteins at the intact proteoform level in complex biological samples to understand proteoform function and cellular mechanisms. However, analysing complex biological samples using TDP is still challenging due to high sample complexity and wide dynamic range. High‐resolution separation methods are often applied prior to mass spectrometry (MS) analysis to decrease sample complexity and increase proteomics throughput. These separation methods, however, may not be efficient enough to characterise low abundance intact proteoforms in complex samples. As such, multidimensional separation techniques (combination of two or more separation methods with high orthogonality) have been developed and applied that demonstrate improved separation resolution and more comprehensive identification in TDP. A suite of multidimensional separation methods that couple various types of liquid chromatography (LC), capillary electrophoresis (CE), and/or gel electrophoresis‐based separation approaches have been developed and applied in TDP to analyse complex biological samples. Here, we reviewed multidimensional separation strategies employed for TDP, summarised current applications, and discussed the gaps that may be addressed in the future.

Journal

Analytical Science AdvancesWiley

Published: Jul 1, 2023

Keywords: CE; gel electrophoresis; LC; multidimensional separation; top–down proteomics

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