Access the full text.
Sign up today, get DeepDyve free for 14 days.
J Chen, BM Balgley, DL DeVoe, CS Lee (2003)
Capillary isoelectric focusing‐based multidimensional concentration/separation platform for proteome analysis, 75
D. Orton, M. Wall, A. Doucette (2013)
Dual LC-MS platform for high-throughput proteome analysis.Journal of proteome research, 12 12
J. Tran, A. Doucette (2008)
Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation.Analytical chemistry, 80 5
J. Carroll, I. Fearnley, J. Walker (2006)
Definition of the mitochondrial proteome by measurement of molecular masses of membrane proteinsProceedings of the National Academy of Sciences, 103
Elijah McCool, Tian Xu, Wenrong Chen, Nicole Beller, Scott Nolan, A. Hummon, Xiaowen Liu, Liangliang Sun (2022)
Deep top-down proteomics revealed significant proteoform-level differences between metastatic and nonmetastatic colorectal cancer cellsScience Advances, 8
Yueju Wang, B. Fonslow, Catherine Wong, A. Nakorchevsky, J. Yates (2012)
Improving the comprehensiveness and sensitivity of sheathless capillary electrophoresis-tandem mass spectrometry for proteomic analysis.Analytical chemistry, 84 20
Yufeng Shen, N. Tolić, P. Piehowski, Anil Shukla, Sangtae Kim, R. Zhao, Yi Qu, E. Robinson, Richard Smith, L. Paša-Tolić (2017)
High-resolution ultrahigh-pressure long column reversed-phase liquid chromatography for top-down proteomics.Journal of chromatography. A, 1498
A. Chambers, J. Mellors, W. Henley, J. Ramsey (2011)
Monolithic integration of two-dimensional liquid chromatography-capillary electrophoresis and electrospray ionization on a microfluidic device.Analytical chemistry, 83 3
Yehia Baghdady, K. Schug (2019)
An Online Comprehensive High pH Reversed Phase x Low pH Reversed Phase Approach for Two-Dimensional Separations of Intact Proteins in Top-Down Proteomics.Analytical chemistry
Xiaojing Shen, Qiang Kou, Ruiqiong Guo, Zhichang Yang, Daoyang Chen, Xiaowen Liu, H. Hong, Liangliang Sun (2018)
Native Proteomics in Discovery Mode Using Size-Exclusion Chromatography-Capillary Zone Electrophoresis-Tandem Mass Spectrometry.Analytical chemistry, 90 17
JJ Pesavento, CR Bullock, RD LeDuc, CA Mizzen, NL Kelleher (2008)
Combinatorial modification of human histone H4 quantitated by two‐dimensional liquid chromatography coupled with top down mass spectrometry, 283
Zachery Gregorich, Ying Ge (2014)
Top‐down proteomics in health and disease: Challenges and opportunitiesPROTEOMICS, 14
D Wessel, UI Flügge (1984)
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids, 138
Jens Hühner, Kevin Jooss, C. Neusüß (2017)
Interference‐free mass spectrometric detection of capillary isoelectric focused proteins, including charge variants of a model monoclonal antibodyELECTROPHORESIS, 38
John Savaryn, Timothy Toby, Adam Catherman, Ryan Fellers, Richard LeDuc, P. Thomas, J. Friedewald, D. Salomon, M. Abecassis, N. Kelleher (2016)
Comparative top down proteomics of peripheral blood mononuclear cells from kidney transplant recipients with normal kidney biopsies or acute rejectionPROTEOMICS, 16
RD Melani, OS Skinner, L Fornelli, GB Domont, PD Compton, NL Kelleher (2016)
Mapping proteoforms and protein complexes from king cobra venom using both denaturing and native top‐down proteomics, 15
Leah Schaffer, R. Millikin, Rachel Miller, Lissa Anderson, Ryan Fellers, Ying Ge, N. Kelleher, Richard LeDuc, Xiaowen Liu, S. Payne, Liangliang Sun, P. Thomas, Trisha Tucholski, Zhe Wang, Si Wu, Zhijie Wu, Dahang Yu, M. Shortreed, Lloyd Smith (2019)
Identification and Quantification of Proteoforms by Mass SpectrometryPROTEOMICS, 19
Xiaojing Shen, Zhichang Yang, Elijah McCool, Rachele Lubeckyj, Daoyang Chen, Liangliang Sun (2019)
Capillary zone electrophoresis-mass spectrometry for top-down proteomics.Trends in analytical chemistry : TRAC, 120
Qingmeng Tang, A. Harrata, Cheng Lee (1995)
Capillary isoelectric focusing-electrospray mass spectrometry for protein analysisAnalytical Chemistry, 67
SB Choi, M Zamarbide, MC Manzini, P Nemes (2017)
Tapered‐tip capillary electrophoresis nano‐electrospray ionization mass spectrometry for ultrasensitive proteomics: the mouse cortex, 28
P. Hemström, K. Irgum (2006)
Hydrophilic interaction chromatography.Journal of separation science, 29 12
R. Burgess (2018)
A brief practical review of size exclusion chromatography: Rules of thumb, limitations, and troubleshooting.Protein expression and purification, 150
J. Kahle, M. Stein, H. Wätzig (2019)
Design of experiments as a valuable tool for biopharmaceutical analysis with (imaged) capillary isoelectric focusingELECTROPHORESIS, 40
Huiming Yuan, Bo Jiang, Baofeng Zhao, L. Zhang, Yukui Zhang (2018)
Recent Advances in Multidimensional Separation for Proteome Analysis.Analytical chemistry, 91 1
Kevin Jooss, N. Scholz, Jens Meixner, C. Neusüß (2019)
Heart‐cut nano‐LC–CZE–MS for the characterization of proteins on the intact levelELECTROPHORESIS, 40
Elijah McCool, Rachele Lubeckyj, Xiaojing Shen, Daoyang Chen, Qiang Kou, Xiaowen Liu, Liangliang Sun (2018)
Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome.Analytical chemistry, 90 9
Kyle Brown, Jake Melby, David Roberts, Ying Ge (2020)
Top-down proteomics: challenges, innovations, and applications in basic and clinical researchExpert Review of Proteomics, 17
J. Mellors, William Black, Andrew Chambers, Jason Starkey, Nathan Lacher, J. Ramsey (2013)
Hybrid capillary/microfluidic system for comprehensive online liquid chromatography-capillary electrophoresis-electrospray ionization-mass spectrometry.Analytical chemistry, 85 8
H Lindner, J Wesierska‐Gadek, W Helliger, B Puschendorf, G Sauermann (1989)
Identification of ADP‐ribosylated histones by the combined use of high‐performance liquid chromatography and electrophoresis, 472
Nertila Siuti, N. Kelleher (2007)
Decoding protein modifications using top-down mass spectrometryNature Methods, 4
A. Vellaichamy, J. Tran, Adam Catherman, J. Lee, John Kellie, S. Sweet, Leonid Zamdborg, P. Thomas, Dorothy Ahlf, Kenneth Durbin, G. Valaskovic, N. Kelleher (2010)
Size-sorting combined with improved nanocapillary liquid chromatography-mass spectrometry for identification of intact proteins up to 80 kDa.Analytical chemistry, 82 4
Tian Xu, Linjie Han, Alayna Thompson, Liangliang Sun (2021)
An improved capillary isoelectric focusing-mass spectrometry method for high-resolution characterization of monoclonal antibody charge variants.Analytical methods : advancing methods and applications
Isabelle François, K. Sandra, P. Sandra (2009)
Comprehensive liquid chromatography: fundamental aspects and practical considerations--a review.Analytica chimica acta, 641 1-2
Kyle Brown, Trisha Tucholski, A. Alpert, Christian Eken, Lucas Wesemann, Andreas Kyrvasilis, Song Jin, Ying Ge (2020)
Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry.Analytical chemistry
Hien Nguyen, K. Schug (2008)
The advantages of ESI-MS detection in conjunction with HILIC mode separations: Fundamentals and applications.Journal of separation science, 31 9
Lloyd Smith, N. Kelleher (2018)
Proteoforms as the next proteomics currencyScience, 359
M. Gama, R. Silva, C. Collins, C. Bottoli (2012)
Hydrophilic interaction chromatographyTrends in Analytical Chemistry, 37
S. Hengel, Erica Floyd, E. Baker, R. Zhao, Si Wu, L. Paša-Tolić (2012)
Evaluation of SDS depletion using an affinity spin column and IMS‐MS detectionPROTEOMICS, 12
Tian Xu, Xiaojing Shen, Zhichang Yang, Daoyang Chen, Rachele Lubeckyj, Elijah McCool, Liangliang Sun (2020)
Automated Capillary Isoelectric Focusing-Tandem Mass Spectrometry for Qualitative and Quantitative Top-Down Proteomics.Analytical chemistry
Kellye Cupp-Sutton, Mulin Fang, Siyi Wu (2022)
Separation methods in single-cell proteomics: RPLC or CE?International journal of mass spectrometry, 481
Adam Catherman, Owen Skinner, N. Kelleher (2014)
Top Down proteomics: facts and perspectives.Biochemical and biophysical research communications, 445 4
H Yamamoto, T Manabe, T Okuyama (1989)
Gel permeation chromatography combined with capillary electrophoresis for microanalysis of proteins, 480
KA Cupp‐Sutton, S Wu (2020)
High‐throughput quantitative top–down proteomics, 16
Yi Du, Fanyu Meng, Steven Patrie, Leah Miller, N. Kelleher (2004)
Improved molecular weight-based processing of intact proteins for interrogation by quadrupole-enhanced FT MS/MS.Journal of proteome research, 3 4
Yuexi Wang, Feng Yang, M. Gritsenko, Yingchun Wang, T. Clauss, Tao Liu, Yufeng Shen, M. Monroe, D. Lopez-Ferrer, Theresa Reno, Ronald Moore, R. Klemke, D. Camp, Richard Smith (2011)
Reversed‐phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cellsPROTEOMICS, 11
Yimeng Zhao, Liangliang Sun, Guijie Zhu, N. Dovichi (2016)
Coupling Capillary Zone Electrophoresis to a Q Exactive HF Mass Spectrometer for Top-down Proteomics: 580 Proteoform Identifications from Yeast.Journal of proteome research, 15 10
R. Melani, V. Gerbasi, Lissa Anderson, J. Sikora, Timothy Toby, Josiah Hutton, D. Butcher, Fernanda Negrão, H. Seckler, K. Srzentić, L. Fornelli, J. Camarillo, Richard LeDuc, Anthony Cesnik, E. Lundberg, Joseph Greer, Ryan Fellers, Matthew Robey, Caroline DeHart, E. Forte, C. Hendrickson, Susan Abbatiello, P. Thomas, A. Kokaji, J. Levitsky, N. Kelleher (2022)
The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cellsScience, 375
I. Ventouri, Daniel Malheiro, Robert Voeten, S. Kok, M. Honing, G. Somsen, R. Haselberg (2020)
Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass SpectrometryAnalytical Chemistry, 92
N. Kelleher, P. Thomas, Ioanna Ntai, Philip Compton, Richard LeDuc (2014)
Deep and quantitative top-down proteomics in clinical and translational researchExpert Review of Proteomics, 11
H Lindner, B Sarg, W Helliger (1997)
Application of hydrophilic‐interaction liquid chromatography to the separation of phosphorylated H1 histones, 782
W Melander, C Horváth (1977)
Salt effects on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic series, 183
Ayako Takemori, Philipp Kaulich, Liam Cassidy, Nobuaki Takemori, A. Tholey (2022)
Size-Based Proteome Fractionation through Polyacrylamide Gel Electrophoresis Combined with LC-FAIMS-MS for In-Depth Top-Down Proteomics.Analytical chemistry
A. Armirotti, G. Damonte (2010)
Achievements and perspectives of top‐down proteomicsPROTEOMICS, 10
C. Mant, R. Hodges (2008)
Mixed-mode hydrophilic interaction/cation-exchange chromatography (HILIC/CEX) of peptides and proteins.Journal of separation science, 31 15
PA Kler, D Sydes, C Huhn (2015)
Column–coupling strategies for multidimensional electrophoretic separation techniques, 407
J. Lee, John Kellie, J. Tran, J. Tipton, Adam Catherman, H. Thomas, Dorothy Ahlf, Kenneth Durbin, A. Vellaichamy, Ioanna Ntai, A. Marshall, N. Kelleher (2009)
A robust two-dimensional separation for top-down tandem mass spectrometry of the low-mass proteomeJournal of the American Society for Mass Spectrometry, 20
N. Kelleher (2004)
Peer Reviewed: Top-Down ProteomicsAnalytical Chemistry, 76
Liam Cassidy, A. Helbig, Philipp Kaulich, Kathrin Weidenbach, R. Schmitz, A. Tholey (2020)
Multidimensional separation schemes enhance the identification and molecular characterization of low molecular weight proteomes and short open reading frame-encoded peptides in top-down proteomics.Journal of proteomics
J. Tran, Leonid Zamdborg, Dorothy Ahlf, J. Lee, Adam Catherman, Kenneth Durbin, J. Tipton, A. Vellaichamy, John Kellie, Mingxi Li, Cong Wu, S. Sweet, Bryan Early, Nertila Siuti, Richard LeDuc, Philip Compton, P. Thomas, N. Kelleher (2011)
Mapping Intact Protein Isoforms in Discovery Mode Using Top Down ProteomicsNature, 480
P. Cutler (1996)
Size-exclusion chromatography.Methods in molecular biology, 59
S Hjertén (1973)
Some general aspects of hydrophobic interaction chromatography, 87
Fanyu Meng, B. Cargile, Steven Patrie, Jeffrey Johnson, Shaun McLoughlin, N. Kelleher (2002)
Processing complex mixtures of intact proteins for direct analysis by mass spectrometry.Analytical chemistry, 74 13
A Jungbauer, R Hahn (2009:)
Methods in Enzymology, 463
K Jooß, J Hühner, S Kiessig, B Moritz, C Neusüß (2017)
Two‐dimensional capillary zone electrophoresis–mass spectrometry for the characterization of intact monoclonal antibody charge variants, including deamidation products, 409
Wenxuan Cai, Trisha Tucholski, Bifan Chen, A. Alpert, S. McIlwain, T. Kohmoto, Song Jin, Ying Ge (2017)
Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy.Analytical chemistry, 89 10
TK Toby, L Fornelli, NL Kelleher (2016)
Progress in top‐down proteomics and the analysis of proteoforms, 9
Lichen Xiu, Santosh Valeja, A. Alpert, Song Jin, Ying Ge (2014)
Effective Protein Separation by Coupling Hydrophobic Interaction and Reverse Phase Chromatography for Top-down ProteomicsAnalytical Chemistry, 86
B. Pirok, D. Stoll, P. Schoenmakers (2018)
Recent Developments in Two-Dimensional Liquid Chromatography: Fundamental Improvements for Practical ApplicationsAnalytical Chemistry, 91
B Buszewski, S Noga (2012)
Hydrophilic interaction liquid chromatography (HILIC) —a powerful separation technique, 402
D. Simpson, Seonghee Ahn, L. Paša-Tolić, B. Bogdanov, H. Mottaz, A. Vilkov, G. Anderson, M. Lipton, Richard Smith (2006)
Using size exclusion chromatography‐RPLC and RPLC‐CIEF as two‐dimensional separation strategies for protein profilingELECTROPHORESIS, 27
G. Reid, S. McLuckey (2002)
'Top down' protein characterization via tandem mass spectrometry.Journal of mass spectrometry : JMS, 37 7
AJ Alpert (1990)
Hydrophilic‐interaction chromatography for the separation of peptides, nucleic acids and other polar compounds, 499
Z Tian, N Tolić, R Zhao (2012)
Enhanced top–down characterization of histone post‐translational modifications, 13
Philip Compton, Leonid Zamdborg, P. Thomas, N. Kelleher (2011)
On the scalability and requirements of whole protein mass spectrometry.Analytical chemistry, 83 17
Z Zhang, S Wu, DL Stenoien, L Paša‐Tolić (2014)
High‐throughput proteomics, 7
Jake Melby, David Roberts, Eli Larson, Kyle Brown, Elizabeth Bayne, Song Jin, Ying Ge (2021)
Novel Strategies to Address the Challenges in Top-Down Proteomics.Journal of the American Society for Mass Spectrometry
Lushuang Huang, Mulin Fang, Kellye Cupp-Sutton, Zhe Wang, Kenneth Smith, Si Wu (2021)
Spray-Capillary-Based Capillary Electrophoresis Mass Spectrometry for Metabolite Analysis in Single Cells.Analytical chemistry
F. Gomes, J. Yates (2019)
Recent trends of capillary electrophoresis-mass spectrometry in proteomics research.Mass spectrometry reviews
EN McCool, JM Lodge, AR Basharat, X Liu, JJ Coon, L Sun (2019)
Capillary zone electrophoresis‐tandem mass spectrometry with activated ion electron transfer dissociation for large‐scale top–down proteomics, 30
Konrad Winkels, T. Koudelka, A. Tholey (2021)
Quantitative Top-Down Proteomics by Isobaric Labeling with Thiol-Directed Tandem Mass Tags.Journal of proteome research
F Hosp, RA Scheltema, HC Eberl (2015)
A double‐barrel liquid chromatography–tandem mass spectrometry (LC–MS/MS) system to quantify 96 interactomes per day*, 14
Xuemei Han, Yueju Wang, A. Aslanian, M. Bern, M. Lavallée-Adam, J. Yates (2014)
Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry for Top-Down Characterization of Pyrococcus furiosus Proteins on a Proteome ScaleAnalytical Chemistry, 86
E. Domínguez-Vega, S. Tengattini, Claudia Peintner, Jordy Angeren, C. Temporini, R. Haselberg, G. Massolini, G. Somsen (2018)
High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry.Talanta, 184
D Mohan, CS Lee (2002)
On‐line coupling of capillary isoelectric focusing with transient isotachophoresis‐zone electrophoresis: a two‐dimensional separation system for proteomics, 23
Dahang Yu, Zhe Wang, Kellye Cupp-Sutton, Yanting Guo, Qiang Kou, Kenneth Smith, Xiaowen Liu, Si Wu (2021)
Quantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling.Journal of the American Society for Mass Spectrometry
Lushuang Huang, Zhe Wang, Kellye Cupp-Sutton, Kenneth Smith, Si Wu (2019)
Spray-Capillary: An Electrospray Assisted Device for Quantitative Ultra-Low Volume Sample Handling.Analytical chemistry
F. Kohl, L. Sánchez‐Hernández, C. Neusüß (2015)
Capillary electrophoresis in two‐dimensional separation systems: Techniques and applicationsELECTROPHORESIS, 36
Ayako Takemori, D. Butcher, V. Harman, Philip Brownridge, Keisuke Shima, D. Higo, J. Ishizaki, Hitoshi Hasegawa, Junpei Suzuki, M. Yamashita, J. Loo, R. Loo, R. Beynon, Lissa Anderson, Nobuaki Takemori (2020)
PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry.Journal of proteome research
Babu Antharavally, Krishna Mallia, M. Rosenblatt, A. Salunkhe, J. Rogers, P. Haney, Navid Haghdoost (2011)
Efficient removal of detergents from proteins and peptides in a spin column format.Analytical biochemistry, 416 1
R. Aebersold, J. Agar, I. Amster, M. Baker, C. Bertozzi, Emily Boja, C. Costello, B. Cravatt, C. Fenselau, B. Garcia, Ying Ge, J. Gunawardena, R. Hendrickson, P. Hergenrother, C. Huber, A. Ivanov, O. Jensen, M. Jewett, N. Kelleher, L. Kiessling, N. Krogan, M. Larsen, J. Loo, R. Loo, E. Lundberg, E. Lundberg, M. MacCoss, P. Mallick, V. Mootha, M. Mrksich, T. Muir, Steven Patrie, James Pesavento, Sharon Pitteri, H. Rodriguez, A. Saghatelian, W. Sandoval, H. Schlüter, S. Sechi, Sarah Slavoff, Lloyd Smith, M. Snyder, P. Thomas, M. Uhlén, J. Eyk, M. Vidal, D. Walt, F. White, E. Williams, T. Wohlschlager, V. Wysocki, N. Yates, N. Young, Bing Zhang (2018)
How many human proteoforms are there?Nature chemical biology, 14 3
NL Young, PA DiMaggio, MD Plazas‐Mayorca, RC Baliban, CA Floudas, BA Garcia (2009)
High throughput characterization of combinatorial histone codes, 8
Philipp Kaulich, Liam Cassidy, A. Tholey (2023)
Identification of proteoforms by top-down proteomics using two-dimensional low/low pH reversed-phase liquid chromatography-mass spectrometry.Proteomics
Kei Webber, Thy Truong, S. Johnston, S. Zapata, Yiran Liang, Jacob Davis, Alexander Buttars, Fletcher Smith, Hailey Jones, Arianna Mahoney, Richard Carson, Andikan Nwosu, Jacob Heninger, A. Liyu, G. Nordin, Ying Zhu, R. Kelly (2022)
Label-Free Profiling of up to 200 Single-Cell Proteomes per Day Using a Dual-Column Nanoflow Liquid Chromatography Platform.Analytical chemistry
M. Pepaj, S. Wilson, K. Novotná, E. Lundanes, T. Greibrokk (2006)
Two-dimensional capillary liquid chromatography: pH gradient ion exchange and reversed phase chromatography for rapid separation of proteins.Journal of chromatography. A, 1120 1-2
Zhe Wang, Dahang Yu, Kellye Cupp-Sutton, Xiaowen Liu, Kenneth Smith, Si Wu (2020)
Development of an Online 2D Ultra-High-Pressure Nano-LC System for High-pH and Low-pH Reversed Phase Separation in Top-Down Proteomics.Analytical chemistry
Naomi Kuehnbaum, Aleshia Kormendi, P. Britz‐McKibbin (2013)
Multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform for metabolomics with high data fidelity.Analytical chemistry, 85 22
M. Bushey, James Jorgenson (1990)
Automated instrumentation for comprehensive two-dimensional high-performance liquid chromatography of proteins.Analytical chemistry, 62 2
F. Gomes, J. Diedrich, Anthony Saviola, E. Memili, A. Moura, J. Yates (2020)
EThcD and 213 nm UVPD for Top-Down Analysis of Bovine Seminal Plasma Proteoforms on Electrophoretic and Chromatographic Time Frames.Analytical chemistry
Bifan Chen, Kyle Brown, Ziqing Lin, Ying Ge (2018)
Top-Down Proteomics: Ready for Prime Time?Analytical chemistry, 90 1
Lloyd Smith, N. Kelleher (2013)
Proteoform: a single term describing protein complexityNature Methods, 10
Elijah McCool, Rachele Lubeckyj, Xiaojing Shen, Qiang Kou, Xiaowen Liu, Liangliang Sun (2018)
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry.Journal of visualized experiments : JoVE, 140
Wenxuan Cai, Trisha Tucholski, Zachery Gregorich, Ying Ge (2016)
Top-down Proteomics: Technology Advancements and Applications to Heart DiseasesExpert Review of Proteomics, 13
E. Livesay, Keqi Tang, Beverley Taylor, Michael Buschbach, D. Hopkins, B. LaMarche, R. Zhao, Yufeng Shen, D. Orton, Ronald Moore, R. Kelly, H. Udseth, Richard Smith (2008)
Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses.Analytical chemistry, 80 1
Hong Wang, S. Hanash (2008)
Increased throughput and reduced carryover of mass spectrometry-based proteomics using a high-efficiency nonsplit nanoflow parallel dual-column capillary HPLC system.Journal of proteome research, 7 7
Alexander Stolz, Kevin Jooss, Oliver Höcker, Jennifer Römer, Johannes Schlecht, C. Neusüß (2018)
Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applicationsELECTROPHORESIS, 40
Yihan Li, Philip Compton, J. Tran, Ioanna Ntai, N. Kelleher (2014)
Optimizing capillary electrophoresis for top‐down proteomics of 30–80 kDa proteinsPROTEOMICS, 14
JA Queiroz, CT Tomaz, JMS Cabral (2001)
Hydrophobic interaction chromatography of proteins, 87
Yanting Guo, Trishika Chowdhury, Meena Seshadri, Kellye Cupp-Sutton, Qingyu Wang, Dahang Yu, Siyi Wu (2023)
Optimization of Higher-Energy Collisional Dissociation Fragmentation Energy for Intact Protein-Level Tandem Mass Tag Labeling.Journal of proteome research
Bifan Chen, Ying Peng, Santosh Valeja, Lichen Xiu, A. Alpert, Ying Ge (2016)
Online Hydrophobic Interaction Chromatography-Mass Spectrometry for Top-Down Proteomics.Analytical chemistry, 88 3
W Cui, HW Rohrs, ML Gross (2011)
Top‐down mass spectrometry: recent developments, applications and perspectives, 136
K. Lorenzatto, Kyunggon Kim, Ioanna Ntai, G. Paludo, J. Lima, P. Thomas, N. Kelleher, H. Ferreira (2015)
Top Down Proteomics Reveals Mature Proteoforms Expressed in Subcellular Fractions of the Echinococcus granulosus Preadult Stage.Journal of proteome research, 14 11
A Stolz, C Neusüß (2022)
Characterisation of a new online nanoLC–CZE–MS platform and application for the glycosylation profiling of alpha‐1‐acid glycoprotein, 414
Santosh Valeja, Lichen Xiu, Zachery Gregorich, Huseyin Guner, Song Jin, Ying Ge (2015)
Three dimensional liquid chromatography coupling ion exchange chromatography/hydrophobic interaction chromatography/reverse phase chromatography for effective protein separation in top-down proteomics.Analytical chemistry, 87 10
Xin Chen, Ying Ge (2013)
Ultrahigh pressure fast size exclusion chromatography for top‐down proteomicsPROTEOMICS, 13
Z. Tian, R. Zhao, N. Tolić, Ronald Moore, D. Stenoien, E. Robinson, Richard Smith, L. Paša-Tolić (2010)
Two‐dimensional liquid chromatography system for online top‐down mass spectrometryPROTEOMICS, 10
D. Botelho, M. Wall, Douglas Vieira, S. Fitzsimmons, Fang Liu, A. Doucette (2010)
Top-down and bottom-up proteomics of SDS-containing solutions following mass-based separation.Journal of proteome research, 9 6
J Hühner, C Neusüß (2016)
CIEF–CZE–MS applying a mechanical valve, 408
EN McCool, D Chen, W Li, Y Liu, L Sun (2019)
Capillary zone electrophoresis‐tandem mass spectrometry with ultraviolet photodissociation (213 Nm) for large‐scale top–down proteomics, 11
Zhe Wang, HongYan Ma, Kenneth Smith, Si Wu (2017)
Two-Dimensional Separation Using High-pH and Low-pH Reversed Phase Liquid Chromatography for Top-down Proteomics.International journal of mass spectrometry, 427
B. Garcia, James Pesavento, C. Mizzen, N. Kelleher (2007)
Pervasive combinatorial modification of histone H3 in human cellsNature Methods, 4
Hangyeore Lee, Jung Lee, Hokeun Kim, Sujin Kim, Jingi Bae, Hark Kim, Sang-Won Lee (2014)
A fully automated dual-online multifunctional ultrahigh pressure liquid chromatography system for high-throughput proteomics analysis.Journal of chromatography. A, 1329
AD Catherman, KR Durbin, DR Ahlf (2013)
Large‐scale top‐down proteomics of the human proteome: membrane proteins, mitochondria, and senescence, 12
KA Cupp‐Sutton, Z Wang, D Yu, S Wu (2022)
Proteoform Identification: Methods and Protocols
John Savaryn, Adam Catherman, P. Thomas, M. Abecassis, N. Kelleher (2013)
The emergence of top-down proteomics in clinical researchGenome Medicine, 5
J. Tran, A. Doucette (2009)
Multiplexed size separation of intact proteins in solution phase for mass spectrometry.Analytical chemistry, 81 15
Andrea Gargano, J. Shaw, Mowei Zhou, Christopher Wilkins, T. Fillmore, Ronald Moore, G. Somsen, L. Paša-Tolić (2018)
Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMSJournal of Proteome Research, 17
Seema Sharma, D. Simpson, N. Tolić, N. Jaitly, A. Mayampurath, Richard Smith, L. Paša-Tolić (2007)
Proteomic profiling of intact proteins using WAX-RPLC 2-D separations and FTICR mass spectrometry.Journal of proteome research, 6 2
D Yu, Z Wang, KA Cupp‐Sutton, X Liu, S Wu (2019)
Deep intact proteoform characterization in human cell lysate using high‐PH and low‐PH reversed‐phase liquid chromatography, 30
Yanting Guo, Dahang Yu, Kellye Cupp-Sutton, Xiaowen Liu, Si Wu (2022)
Optimization of protein-level tandem mass tag (TMT) labeling conditions in complex samples with top-down proteomics.Analytica chimica acta, 1221
Kenneth Durbin, L. Fornelli, Ryan Fellers, P. Doubleday, M. Narita, N. Kelleher (2016)
Quantitation and Identification of Thousands of Human Proteoforms below 30 kDa.Journal of proteome research, 15 3
Jiangtao Ren, Matthew Beckner, Kyle Lynch, Huang Chen, Zaifang Zhu, Yu Yang, Apeng Chen, Zhenzhen Qiao, Shaorong Liu, J. Lu (2018)
Two-dimensional liquid chromatography consisting of twelve second-dimension columns for comprehensive analysis of intact proteins.Talanta, 182
Bryon Drown, Kevin Jooss, R. Melani, Cameron Lloyd-Jones, J. Camarillo, Neil Kelleher (2022)
Mapping the Proteoform Landscape of Five Human TissuesJournal of Proteome Research, 21
ÖB Acikara, ÖB Acikara (2013)
Ion‐Exchange Chromatography and its Applications
Zhijie Wu, Hongxia Wang, Andrew Tustian, Haibo Qiu, Ning Li (2022)
Development of a Two-Dimensional Liquid Chromatography-Mass Spectrometry Platform for Simultaneous Multi-Attribute Characterization of Adeno-Associated Viruses.Analytical chemistry
H Lee, D‐G Mun, J Bae (2015)
A simple dual online ultra‐high pressure liquid chromatography system (SDO‐UHPLC) for high throughput proteome analysis, 140
Top–down proteomics (TDP) identifies, quantifies, and characterises proteins at the intact proteoform level in complex biological samples to understand proteoform function and cellular mechanisms. However, analysing complex biological samples using TDP is still challenging due to high sample complexity and wide dynamic range. High‐resolution separation methods are often applied prior to mass spectrometry (MS) analysis to decrease sample complexity and increase proteomics throughput. These separation methods, however, may not be efficient enough to characterise low abundance intact proteoforms in complex samples. As such, multidimensional separation techniques (combination of two or more separation methods with high orthogonality) have been developed and applied that demonstrate improved separation resolution and more comprehensive identification in TDP. A suite of multidimensional separation methods that couple various types of liquid chromatography (LC), capillary electrophoresis (CE), and/or gel electrophoresis‐based separation approaches have been developed and applied in TDP to analyse complex biological samples. Here, we reviewed multidimensional separation strategies employed for TDP, summarised current applications, and discussed the gaps that may be addressed in the future.
Analytical Science Advances – Wiley
Published: Jul 1, 2023
Keywords: CE; gel electrophoresis; LC; multidimensional separation; top–down proteomics
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.