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Quantitation of low molecular weight sugars by chemical derivatization‐liquid chromatography/multiple reaction monitoring/mass spectrometry

Quantitation of low molecular weight sugars by chemical derivatization‐liquid... A new method for the separation and quantitation of 13 mono‐ and disaccharides has been developed by chemical derivatization/ultra‐HPLC/negative‐ion ESI‐multiple‐reaction monitoring MS. 3‐Nitrophenylhydrazine (at 50°C for 60 min) was shown to be able to quantitatively derivatize low‐molecular weight (LMW) reducing sugars. The nonreducing sugar, sucrose, was not derivatized. A pentafluorophenyl‐bonded phase column was used for the chromatographic separation of the derivatized sugars. This method exhibits femtomole‐level sensitivity, high precision (CVs of ≤ 4.6%) and high accuracy for the quantitation of LMW sugars in wine. Excellent linearity (R2 ≥ 0.9993) and linear ranges of ∼500‐fold for disaccharides and ∼1000–4000‐fold for monosaccharides were achieved. With internal calibration (13C‐labeled internal standards), recoveries were between 93.6% ± 1.6% (xylose) and 104.8% ± 5.2% (glucose). With external calibration, recoveries ranged from 82.5% ± 0.8% (ribulose) to 105.2% ± 2.1% (xylulose). Quantitation of sugars in two red wines and two white wines was performed using this method; quantitation of the central carbon metabolism‐related carboxylic acids and tartaric acid was carried out using a previously established derivatization procedure with 3‐nitrophenylhydrazine as well. The results showed that these two classes of compounds—both of which have important organoleptic properties—had different compositions in red and white wines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Electrophoresis Wiley

Quantitation of low molecular weight sugars by chemical derivatization‐liquid chromatography/multiple reaction monitoring/mass spectrometry

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References (23)

Publisher
Wiley
Copyright
"© 2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim"
ISSN
0173-0835
eISSN
1522-2683
DOI
10.1002/elps.201600150
pmid
27120558
Publisher site
See Article on Publisher Site

Abstract

A new method for the separation and quantitation of 13 mono‐ and disaccharides has been developed by chemical derivatization/ultra‐HPLC/negative‐ion ESI‐multiple‐reaction monitoring MS. 3‐Nitrophenylhydrazine (at 50°C for 60 min) was shown to be able to quantitatively derivatize low‐molecular weight (LMW) reducing sugars. The nonreducing sugar, sucrose, was not derivatized. A pentafluorophenyl‐bonded phase column was used for the chromatographic separation of the derivatized sugars. This method exhibits femtomole‐level sensitivity, high precision (CVs of ≤ 4.6%) and high accuracy for the quantitation of LMW sugars in wine. Excellent linearity (R2 ≥ 0.9993) and linear ranges of ∼500‐fold for disaccharides and ∼1000–4000‐fold for monosaccharides were achieved. With internal calibration (13C‐labeled internal standards), recoveries were between 93.6% ± 1.6% (xylose) and 104.8% ± 5.2% (glucose). With external calibration, recoveries ranged from 82.5% ± 0.8% (ribulose) to 105.2% ± 2.1% (xylulose). Quantitation of sugars in two red wines and two white wines was performed using this method; quantitation of the central carbon metabolism‐related carboxylic acids and tartaric acid was carried out using a previously established derivatization procedure with 3‐nitrophenylhydrazine as well. The results showed that these two classes of compounds—both of which have important organoleptic properties—had different compositions in red and white wines.

Journal

ElectrophoresisWiley

Published: Jul 1, 2016

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