Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Simultaneous chiral resolution of monosaccharides as 8‐aminonaphthalene‐1,3,6‐trisulfonate derivatives by ligand‐exchange CE using borate as a central ion of the chiral selector

Simultaneous chiral resolution of monosaccharides as 8‐aminonaphthalene‐1,3,6‐trisulfonate... Six reducing monosaccharides (mannose, galactose, fucose, glucose, xylose, and arabinose) were derivatized with 8‐aminonaphthalene‐1,3,6‐trisulfonate (ANTS). Based on the chiral ligand‐exchange principle using borate as a central ion of the chiral selector and (S)‐3‐amino‐1,2‐propanediol (SAP) as a chiral selector ligand, all of the six ANTS‐monosaccharides were simultaneously enantioseparated using absorbance at 245 nm for detection. The optimum conditions for both high resolution and moderately short migration time consisted of 200 mM SAP–200 mM borate buffer (pH 9.2) containing 10% ACN as a BGE at 30°C with an applied voltage of +30 kV. It was revealed that the proposed chiral ligand‐exchange CE using the SAP–borate system was applicable to enantioseparation of not only diols but also polyols. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Electrophoresis Wiley

Simultaneous chiral resolution of monosaccharides as 8‐aminonaphthalene‐1,3,6‐trisulfonate derivatives by ligand‐exchange CE using borate as a central ion of the chiral selector

Loading next page...
 
/lp/wiley/simultaneous-chiral-resolution-of-monosaccharides-as-8-vxVJH9KuSX

References (25)

Publisher
Wiley
Copyright
"Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
0173-0835
eISSN
1522-2683
DOI
10.1002/elps.200700306
pmid
17924372
Publisher site
See Article on Publisher Site

Abstract

Six reducing monosaccharides (mannose, galactose, fucose, glucose, xylose, and arabinose) were derivatized with 8‐aminonaphthalene‐1,3,6‐trisulfonate (ANTS). Based on the chiral ligand‐exchange principle using borate as a central ion of the chiral selector and (S)‐3‐amino‐1,2‐propanediol (SAP) as a chiral selector ligand, all of the six ANTS‐monosaccharides were simultaneously enantioseparated using absorbance at 245 nm for detection. The optimum conditions for both high resolution and moderately short migration time consisted of 200 mM SAP–200 mM borate buffer (pH 9.2) containing 10% ACN as a BGE at 30°C with an applied voltage of +30 kV. It was revealed that the proposed chiral ligand‐exchange CE using the SAP–borate system was applicable to enantioseparation of not only diols but also polyols.

Journal

ElectrophoresisWiley

Published: Nov 1, 2007

Keywords: ; ; ; ;

There are no references for this article.