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Solid‐phase permethylation of glycans for mass spectrometric analysis

Solid‐phase permethylation of glycans for mass spectrometric analysis A miniaturized approach was developed for quantitative permethylation of oligosaccharides, which involves packing of sodium hydroxide powder in microspin columns or fused‐silica capillaries (500 µm i.d.), permitting effective derivatization in less than a minute at microscale. Prior to mass spectrometry, analytes are mixed with methyl iodide in dimethyl sulfoxide solution containing traces of water before infusing through the microreactors. This procedure minimizes oxidative degradation and peeling reactions and avoids the need of excessive clean‐up. Picomole amounts of linear and branched, sialylated and neutral glycan samples were rapidly and efficiently permethylated by this approach and analyzed by matrix‐assisted laser desorption/ionization mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rapid Communications in Mass Spectrometry Wiley

Solid‐phase permethylation of glycans for mass spectrometric analysis

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References (34)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services
ISSN
0951-4198
eISSN
1097-0231
DOI
10.1002/rcm.2210
pmid
16252310
Publisher site
See Article on Publisher Site

Abstract

A miniaturized approach was developed for quantitative permethylation of oligosaccharides, which involves packing of sodium hydroxide powder in microspin columns or fused‐silica capillaries (500 µm i.d.), permitting effective derivatization in less than a minute at microscale. Prior to mass spectrometry, analytes are mixed with methyl iodide in dimethyl sulfoxide solution containing traces of water before infusing through the microreactors. This procedure minimizes oxidative degradation and peeling reactions and avoids the need of excessive clean‐up. Picomole amounts of linear and branched, sialylated and neutral glycan samples were rapidly and efficiently permethylated by this approach and analyzed by matrix‐assisted laser desorption/ionization mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.

Journal

Rapid Communications in Mass SpectrometryWiley

Published: Jan 15, 2005

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